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US 11,913,060 B1Granted
Methods for quantifying mixed-linkage beta glucan
AssigneeEdeniq, Inc.
InventorsPrachand Shrestha, Denmark Antolin
Life of this patent
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Abstract
Methods and solutions are disclosed for the quantification of mixed-linkage beta-glucan in plant biomass samples. Improved alkaline pretreatment, enzymatic hydrolysis, and glucose measurement parameters are provided that increase the accuracy, precision, and sensitivity of mixed-linkage beta-glucan assays.
Claims21
A method of accurately quantifying a concentration of mixed-linkage beta-glucan in a plant biomass sample, the method comprising:
- contacting the plant biomass sample with an alkaline mixture thereby generating a pretreated sample, wherein the alkaline mixture comprises sodium hydroxide or potassium hydroxide at a concentration between 0.2 N and 1 N;
- wherein the contacting is at a pretreatment temperature between 10° C. and 35° C. and a pretreatment duration between 20 minutes and less than 3 hours;
- hydrolyzing mixed-linkage beta-glucan in the pretreated sample to generate glucose;
- measuring the concentration of glucose in the pretreated sample;
- and
- transforming the measured glucose concentration to a calculated mixed-linkage beta-glucan concentration by multiplying the measured glucose concentration value by a conversion factor 1.11 g glucose/1 g mixed-linkage beta-glucan, wherein the calculated mixed-linkage beta-glucan concentration accurately quantifies the concentration of mixed-linkage beta-glucan in the plant biomass sample.
3 more elements
A method of accurately quantifying a concentration of mixed-linkage beta-glucan in a plant biomass sample, the method comprising:
- contacting the plant biomass sample with an alkaline mixture thereby generating a pretreated sample, wherein the alkaline mixture comprises a metal hydroxide at a concentration between 0.2 N and 1 N, wherein the contacting is at a pretreatment temperature between 10° C. and 35° C. and a pretreatment duration between 20 minutes and less than 3 hours;
- hydrolyzing mixed-linkage beta-glucan in the pretreated sample to generate glucose;
- measuring the concentration of glucose in the pretreated sample;
- and
- transforming the measured glucose concentration to a calculated mixed-linkage beta-glucan concentration by multiplying the measured glucose concentration value by a conversion factor 1.11 g glucose/1 g mixed-linkage beta-glucan, wherein the calculated mixed-linkage beta-glucan concentration accurately quantifies the concentration of mixed-linkage beta-glucan in the plant biomass sample.
2 more elements
Dependencies are inferred from the claim text.