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US 11,913,053 B2Granted
Application of trehalase in fermentative production
AssigneeNanjing Bestzyme Bio-Engineering Co., Ltd.
InventorsJie Lin, Hongxian Xu, Hui Peng
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Abstract
Provided is an application of trehalase in fermentative production. The trehalase has amino acid sequences shown in SEQ ID NO.6, SEQ ID NO.7, and SEQ ID NO.8. Provided are methods for producing and applying trehalase, particularly being applied in the production and fermentation of alcohol and an amino acid.
Claims20
A method for producing a fermented product comprising an alcohol, wherein the method comprises:
- (a) adding amylase to liquefy an alcohol fermentation raw material;
- (b) saccharifying the liquefied raw material;
- (c) adding yeast and performing fermentation;
- (d) collecting alcohol mature mash supernatant after the end of fermentation;
- and
- (e) adding a polypeptide with trehalase activity to the alcohol mature mash supernatant at an amount of 0.05-10 U/g DS to produce the fermented product, wherein the polypeptide comprises at least 95% sequence identity to the polypeptide of SEQ ID NO:
- 6.
4 more elements
A method for producing a fermented product comprising an amino acid, wherein the method comprises:
- (a) culturing a seed solution of fermentation microbes;
- (b) performing fermentation;
- (c) collecting a fermentation solution;
- and
- (d) adding a polypeptide with trehalase activity to the fermentation solution at a concentration of 0.05-5 U/ml and reacting the fermentation solution comprising the polypeptide with trehalase activity at about 37° C., for about 5 hours, at about pH 6.8 to produce the fermented product, wherein the polypeptide comprises at least 95% sequence identity to the polypeptide of SEQ ID NO:
- 6.
3 more elements
Dependencies are inferred from the claim text.