Back to results
US 11,913,051 B2Granted
Engineered and fully-functional customized glycoproteins
AssigneeLimmatech Biologics AG
InventorsAmirreza Faridmoayer, Manuela Mally, Rainer Follador
Life of this patent
Estimating the term…
Abstract
Described herein are compositions and methods of producing glycosylated proteins in vitro and in vivo. The methods include using host cells to produce glycosylated proteins. Also described herein are glycosylated proteins produced using such methods and uses thereof.
Claims49
A Leishmania host cell comprising:
- a. a recombinant nucleic acid encoding a target protein;
- and
- b. a recombinant nucleic acid encoding a heterologous glycosyltransferase, wherein the heterologous glycosyltransferase is an N-acetyl glucosamine transferase that is not from Leishmania and/or galactosyltransferase that is not from Leishmania;
- wherein the heterologous glycosyltransferase comprises a signal and/or retention sequence, wherein the signal sequence is capable of targeting the heterologous glycosyltransferase to the secretory pathway of the Leishmania host cell, and wherein the retention sequence is capable of retaining the heterologous glycosyltransferase in the Golgi apparatus.
1 more element
Dependencies are inferred from the claim text.