Methods for the modification of peptides harboring an N-terminus glycine residue
AssigneesCentre National De La Recherche Scientifique, Commissariat A L'energie Atomique Et Aux Energies Alternatives, Universite Grenoble Alpes
InventorsBruno Franzetti, Eric Girard, Alexandre Appolaire, Hind Basbous
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Abstract
The invention relates to the uses of a new characterized TET protein showed restricted to N-terminus glycine residues exopeptidase. The invention also relates to a method comprising said use of said new characterized TET protein as a N-terminus glycine residues specific exopeptidase. The invention further relates to a support wherein it is immobilized on said new characterized TET protein as a N-terminus glycine residues specific exopeptidase.
Claims14
A method for the modification of all or part of the polypeptide content of a substrate comprising peptides, polypeptides and/or proteins harbouring a N-terminus glycine residue, comprising contacting all or part of the substrate, peptides, polypeptides and/or proteins harbouring a N-terminus glycine residue, with at least a TET protein harbouring at least a N-terminus glycine residues specific exopeptidase activity, said at least TET protein comprising the amino acid sequence as set forth in SEQ ID NO:
- 1, or with any homologous protein derived from said at least TET protein as set forth in SEQ ID NO:
- 1 by substitution, addition or deletion of at least one amino acid, provided that the derived protein retains at least 79% of identity with the amino acid sequence as set forth in SEQ ID NO:
- 1, and said derived protein retaining a N-terminus glycine residues specific exopeptidase activity.
Dependencies are inferred from the claim text.