Engineered adenylate kinase variant enzymes
AssigneeCodexis, Inc.
InventorsDa Duan, Auric Anthony Sowell-Kantz, Aksiniya Lyubenova Petkova, Oscar Alvizo
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Abstract
The present invention provides engineered adenylate kinase (AdK) enzymes, polypeptides having AdK activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing AdK enzymes are also provided. The present invention further provides compositions comprising the AdK enzymes and methods of using the engineered AdK enzymes. The present invention finds particular use in the production of pharmaceutical compounds.
Claims17
An engineered adenylate kinase comprising a polypeptide sequence having at least 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% sequence identity to SEQ ID NO:
- 4, wherein the polypeptide sequence of said engineered adenylate kinase comprises a substitution at position 42 and wherein the amino acid positions of said polypeptide sequence are numbered with reference to SEQ ID NO:
- 4.
Dependencies are inferred from the claim text.