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US 11,912,980 B2Granted
Method for the production of a dairy food product and a method for gene transfer by conjugation
AssigneeDsm IP Assets B.V.
InventorsMariya Alexeevna Tarazanova, Thom Huppertz, Herwig Bachmann, Jan Kok, Marke Beerthuyzen
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Abstract
The present invention relates to the field of microbiology, specifically the field of production of a dairy food product using fermentation and the field of gene transfer by conjugation.
Claims12
A method for gene transfer by conjugation comprising
- a) providing a composition comprising at least two bacterial strains, wherein at least one bacterial strain comprises an increased amount of expression product of a pilin gene cluster compared to that of Lactococcus lactis subsp. cremoris NCDO 712 when cultured under identical conditions, and
- b) incubating the composition under conditions conducive to conjugation.
A method for the production of a desired bacterial strain comprising a genetic trait of interest, comprising
- a) providing a composition comprising:
- i) a precursor of the desired bacterial strain, wherein the precursor lacks the genetic trait of interest;
- and
- ii) a donor bacterial strain comprising the genetic trait of interest;
- wherein at least one bacterial strain in the composition comprises an increased amount of expression product of a pilin gene cluster compared to that of Lactococcus lactis subsp. cremoris NCDO 712 when cultured under identical conditions;
- b) incubating the composition under conditions conducive to conjugation, and
- c) optionally isolating the desired bacterial strain comprising the desired genetic trait of interest.
4 more elements
Dependencies are inferred from the claim text.