# US 11,910,808 B2 · Ruminant compositions > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,910,808 B2 - Document ID: US11910808B2 - Status: Granted - Kind: B2 - Application number: 16/920,997 - Filed: 2020-07-06 - Published: 2021-02-11 - Granted: 2024-02-27 - Earliest claimed: 2016-01-07 - Assignees: Native Microbials, Inc. - Applicants: Native Microbials, Inc. - Inventors: Mallory Embree; Luke Picking; Grant Gogul; Janna Tarasova - Web page: https://patentgrep.com/patent/US11910808B2 ## CPC - A23K 10/18: of live microorganisms - A23K 10/12: by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass - A23K 10/16: Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions (A23K10/12 takes precedence) - A23K 10/30: from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms (obtained by microbiological or biochemical processes, e.g. using yeasts or enzymes, A23K10/10) - A23K 10/37: from waste material (from hydrolysates of wood or straw A23K10/32; from molasses A23K10/33) - A23K 20/158: Fatty acids; Fats; Products containing oils or fats - A23K 20/163: Sugars; Polysaccharides - A23K 20/28: Silicates, e.g. perlites, zeolites or bentonites - A23K 40/35: Making capsules specially adapted for ruminants - A23K 50/10: for ruminants - A23L 31/10: Yeasts or derivatives thereof - A23L 33/135: Bacteria or derivatives thereof, e.g. probiotics - A61K 9/0019: {Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner (non-active ingredients are additionally classified in A61K47/00)} - A61K 9/0053: {Mouth and digestive tract, i.e. intraoral and peroral administration} - A61K 35/74: Bacteria (therapeutic use of a bacterial protein A61K38/00) - A61K 35/741: Probiotics (probiotic yeast, e.g. saccharomyces A61K36/06) - A61K 35/742: Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes - A61K 36/06: Fungi, e.g. yeasts - A61K 36/062: Ascomycota - A61K 36/064: Saccharomycetales, e.g. baker's yeast - A61K 39/02: Bacterial antigens - A61P 15/04: for inducing labour or abortion; Uterotonics - A61P 31/04: Antibacterial agents - C12N 1/14: Fungi (culture of mushrooms A01G18/00; as new plants A01H15/00); Culture media therefor - C12N 1/145: Fungi isolates - C12N 1/20: Bacteria; Culture media therefor - C12N 1/205: Bacterial isolates - A61K 35/748: Cyanobacteria, i.e. blue-green bacteria or blue-green algae, e.g. spirulina (algae, microalgae or microphytes A61K36/02) - C12R 2001/01: Bacteria or Actinomycetales {; using bacteria or Actinomycetales} - C12R 2001/145: Clostridium - C12R 2001/645: Fungi {; Processes using fungi} - A23K: FODDER - A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B) - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS ## Abstract The present disclosure provides compositions formulated for ruminants that comprise beneficial microbes. Particularly, the disclosure provides a composition comprising microorganisms with at least 97% sequence identity to any one of SEQ ID NOs: 1-60 and 2045-2107. Such compositions can be used for increasing milk production or improving milk compositional characteristics in ruminants. ## Claims ### Claim 1 A method for increasing milk production or improving milk compositional characteristics in a ruminant, the method comprising orally administering to a ruminant an effective amount of a ruminant supplement comprising: a) a purified microbial population that comprises at least one bacterium with a 16S nucleic acid sequence and/or at least one fungus with an ITS nucleic acid sequence, which is at least 97% identical to a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-60 and 2045-2107; and b) a carrier suitable for ruminant administration, and wherein the ruminant administered the effective amount of the ruminant supplement exhibits an increase in milk production or improved milk compositional characteristics, as compared to a ruminant not administered the ruminant supplement. ### Claim 2 (depends on 1) The method according to claim 1, wherein the ruminant is a cow. ### Claim 3 (depends on 1) The method according to claim 1, wherein the ruminant supplement is formulated as an encapsulation, tablet, capsule, pill, feed additive, food ingredient, food additive, food preparation, food supplement, consumable solution, consumable spray additive, consumable solid, consumable gel, bolus, drench, or combinations thereof. ### Claim 4 (depends on 1) The method according to claim 1, wherein the ruminant supplement is encapsulated in a polymer or carbohydrate. ### Claim 5 (depends on 1) The method according to claim 1, wherein administering comprises: feeding the ruminant supplement to a ruminant. ### Claim 6 (depends on 1) The method according to claim 1, wherein the purified microbial population is present in the ruminant supplement in an amount of at least 102 cells. ### Claim 7 (depends on 1) The method according to claim 1, wherein the purified microbial population comprises a bacterium with a 16S nucleic acid sequence that is at least 97% identical to a nucleic acid sequence selected from the group consisting of: SEQ ID NOs: 1-30 and 2045-2103. ### Claim 8 (depends on 1) The method according to claim 1, wherein the purified microbial population comprises a fungus with an ITS nucleic acid sequence that is at least 97% identical to a nucleic acid sequence selected from the group consisting of: SEQ ID NOs: 31-60 and 2104-2107. ### Claim 9 (depends on 1) The method according to claim 1, wherein the purified microbial population comprises a bacterium with a 16S nucleic acid sequence that is at least 99% identical to a nucleic acid sequence selected from the group consisting of: SEQ ID NOs: 1-30 and 2045-2103. ### Claim 10 (depends on 1) The method according to claim 1, wherein the purified microbial population comprises a fungus with an ITS nucleic acid sequence that is at least 99% identical to a nucleic acid sequence selected from the group consisting of: SEQ ID NOs: 31-60 and 2104-2107. ### Claim 11 (depends on 1) The method according to claim 1, wherein the purified microbial population comprises a bacterium with a 16S nucleic acid sequence selected from the group consisting of: SEQ ID NOs: 1-30 and 2045-2103. ### Claim 12 (depends on 1) The method according to claim 1, wherein the purified microbial population comprises a fungus with an ITS nucleic acid sequence selected from the group consisting of: SEQ ID NOs: 31-60 and 2104-2107. ### Claim 13 (depends on 1) The method according to claim 1, wherein the purified microbial population comprises a bacterium with a 16S nucleic acid sequence that is at least 97% identical to SEQ ID NO: 28. ### Claim 14 (depends on 1) The method according to claim 1, wherein the purified microbial population comprises a fungus with an ITS nucleic acid sequence that is at least 97% identical to SEQ ID NO: 32. ### Claim 15 (depends on 1) The method according to claim 1, wherein the purified microbial population comprises a bacteria with a 16S nucleic acid sequence and/or a fungus with an ITS nucleic acid sequence selected from the group consisting of: SEQ ID NOs: 1-60 and 2045-2107. ### Claim 16 (depends on 1) The method according to claim 1, wherein the purified microbial population comprises a bacterium with a 16S nucleic acid sequence of SEQ ID NO: 28. ### Claim 17 (depends on 1) The method according to claim 1, wherein the purified microbial population comprises a fungus with an ITS nucleic acid sequence of SEQ ID NO: 32. ### Claim 18 (depends on 1) The method according to claim 1, wherein the purified microbial population comprises a Pichia fungus as deposited at NRRL Y-67249. ### Claim 19 (depends on 1) The method according to claim 1, wherein the purified microbial population comprises a Clostridium bacterium as deposited at NRRL B-67248. ### Claim 20 (depends on 1) The method according to claim 1, wherein the purified microbial population comprises a bacterium with a 16S nucleic acid sequence of SEQ ID NO: 28 and/or a fungi with an ITS nucleic acid sequence of SEQ ID NO: 32. ### Claim 21 (depends on 1) The method according to claim 1, wherein the purified microbial population comprises a Pichia fungus as deposited at NRRL Y-67249 and/or a Clostridium bacteria as deposited at NRRL B-67248. ### Claim 22 (depends on 1) The method according to claim 1, wherein the ruminant administered the effective amount of the ruminant supplement exhibits an increase in milk production that leads to a measured increase in milk yield. ### Claim 23 (depends on 1) The method according to claim 1, wherein the ruminant administered the effective amount of the ruminant supplement exhibits an increase in milk production and improved milk compositional characteristics that leads to a measured increase in energy-corrected milk. ### Claim 24 (depends on 1) The method according to claim 1, wherein the ruminant administered the effective amount of the ruminant supplement exhibits an improved milk compositional characteristic selected from the group consisting of an increase in milk fat(s), an increase in milk protein(s), an increase of carbohydrates in milk, an increase of vitamins in milk, an increase of minerals in milk, or combinations thereof. ### Claim 25 (depends on 1) The method according to claim 1, wherein the ruminant administered the effective amount of the ruminant supplement exhibits at least a 1% increase in the average production of milk fat(s), milk protein(s), energy-corrected milk, or combinations thereof. ### Claim 26 (depends on 1) The method according to claim 1, wherein the ruminant administered the effective amount of the ruminant supplement exhibits at least a 10% increase in the average production of milk fat(s), milk protein(s), energy-corrected milk, or combinations thereof. ### Claim 27 (depends on 1) The method according to claim 1, wherein the ruminant administered the effective amount of the ruminant supplement exhibits at least a 20% increase in the average production of milk fat(s), milk protein(s), energy-corrected milk, or combinations thereof. ### Claim 28 (depends on 1) The method according to claim 1, wherein the ruminant administered the effective amount of the ruminant supplement, further exhibits: at least one improved phenotypic trait, selected from the group consisting of an improved efficiency in feed utilization, improved digestibility, an increase in polysaccharide and lignin degradation, an increase in fatty acid concentration in the rumen, pH balance in the rumen, a reduction in methane emissions, a reduction in manure production, improved dry matter intake, an improved efficiency of nitrogen utilization, or combinations thereof. ### Claim 29 (depends on 1) The method according to claim 1, wherein the ruminant administered the effective amount of the ruminant supplement, further exhibits: a shift in the microbiome of the rumen. ### Claim 30 (depends on 1) The method according to claim 1, wherein the ruminant administered the effective amount of the ruminant supplement, further exhibits: a shift in the microbiome of the rumen, wherein a population of microbes present in the rumen before administration of the ruminant supplement increase in abundance after administration of the ruminant supplement. ### Claim 31 (depends on 1) The method according to claim 1, wherein the ruminant administered the effective amount of the ruminant supplement, further exhibits: a shift in the microbiome of the rumen, wherein a population of microbes present in the rumen before administration of the ruminant supplement decrease in abundance after administration of the ruminant supplement. ### Claim 32 (depends on 1) The method according to claim 1, wherein the ruminant administered the effective amount of the ruminant supplement, further exhibits: a shift in the microbiome of the rumen, wherein a first population of microbes present in the rumen before administration of the ruminant supplement increase in abundance after administration of the ruminant supplement, and wherein a second population of microbes present in the rumen before administration of the ruminant supplement decrease in abundance after administration of the ruminant supplement. ### Claim 33 (depends on 1) The method according to claim 1, wherein the purified microbial population only contains organisms that are members of a group selected from Intestinimonas, Anaerolinea, Pseudobutyrivibrio, Olsenella, Eubacterium, Catenisphaera, Faecalibacterium, Solobacterium, Blautia, Ralsonia, Coprococcus, Casaltella, Anaeroplasma, Acholeplasma, Aminiphilus, Mitsuokella, Alistipes, Sharpea, Oscillibacter, Neocallimastix, Odoribacter, Pichia, Tannerella, Candida, Hydrogenoanaerobacterium, Orpinomyces, Succinivibrio, Sugiyamaella, Ruminobacter, Lachnospira, Caecomyces, Sinimarinibacterium, Tremella, Hydrogenoanaerobacterium, Turicibacter, Clostridium XIVa, Anaerolinea, Saccharofermentans, Butyricicoccus, Olsenella, Papillibacter, Clostridium XIa, Pelotomaculum, Erysipelotrichaceae incertae sedis, Lachnospiracea incertae sedis, Solobacterium, Anaeroplasma, Ralstonia, Clostridium sensu stricto, Eubacterium, Rikenella, Lachnobacterium, Tannerella, Acholeplasma, Howardella, Selenomonas, Butyricimonas, Sharpea, Succinivibrio, Ruminobacter, Candida, Syntrophococcus, Pseudobutyrivibrio, Orpinomyces, Cyllamyces, Saccharomycetales, Phyllosticta, Ascomycota, and Piromyces. ### Claim 34 (depends on 1) The method according to claim 1, wherein the at least one bacterium or fungus is capable of converting a carbon source into a volatile fatty acid selected from the group consisting of acetate, butyrate, propionate, or combinations thereof. ### Claim 35 (depends on 1) The method according to claim 1, wherein the at least one bacterium or fungus is capable of degrading a soluble or insoluble carbon source.