# US 11,906,722 B2 · Electromagnetic wave determining device, flow cytometer, electromagnetic wave determining method, and electromagnetic wave determining program > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,906,722 B2 - Document ID: US11906722B2 - Status: Granted - Kind: B2 - Application number: 18/056,432 - Filed: 2022-11-17 - Published: 2023-07-06 - Granted: 2024-02-20 - Earliest claimed: 2017-02-17 - Assignees: Osaka University; The University of Tokyo - Applicants: Osaka University; The University of Tokyo - Inventors: Ryoichi Horisaki; Jun Tanida; Sadao Ota - Web page: https://patentgrep.com/patent/US11906722B2 ## CPC - G02B 21/14: affording illumination for phase-contrast observation - G01N 15/14: Optical investigation techniques, e.g. flow cytometry - G01N 15/1434: Optical arrangements - G01N 21/41: Refractivity; Phase-affecting properties, e.g. optical path length (G01N21/21 takes precedence) - G06F 18/214: Generating training patterns; Bootstrap methods, e.g. bagging or boosting - G01N 15/149: specially adapted for sorting particles, e.g. by their size or optical properties - G01N 2015/144: {Imaging characterised by its optical setup} - G01N 2015/145: {by pattern of light, e.g. fringe pattern} - G02B: OPTICAL ELEMENTS, SYSTEMS OR APPARATUS - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - G06F: ELECTRIC DIGITAL DATA PROCESSING (computer systems based on specific computational models G06N) ## Abstract An electromagnetic wave detecting device comprising: an emission unit configured to emit electromagnetic waves having coherence; an electromagnetic wave modulating unit configured to modulate one or both of a phase and an amplitude of the emitted electromagnetic waves and to change a state of the modulation relative to an imaging target; and a post-modulation electromagnetic wave intensity detecting unit configured to detect an intensity of post-modulation electromagnetic waves, which are the modulated electromagnetic waves acquired by modulating the electromagnetic waves emitted from the emission unit using the imaging target and the electromagnetic wave modulating unit, using one pixel. ## Claims ### Claim 1 A device for imaging an object comprising: a flow path configured to permit an object to flow therethrough; an emission unit configured to emit at least one electromagnetic wave having at least partial coherence, wherein the emission unit is configured to emit the at least one electromagnetic wave towards the flow path to cause at least one optical interaction between the at least one electromagnetic wave and the object; an electromagnetic wave modulating unit configured to modulate one or both of a phase or an amplitude of the at least one electromagnetic wave following the at least one optical interaction to produce at least one modulated electromagnetic wave, wherein the electromagnetic wave modulating unit comprises a plurality of areas configured to modulate the at least one electromagnetic wave differently; an electromagnetic wave intensity detecting unit configured to detect at least one intensity of the at least one modulated electromagnetic wave; and a generation unit configured to generate at least a phase image or an amplitude image of the object based at least in part on the at least one intensity of the at least one modulated electromagnetic wave detected by the electromagnetic wave intensity detecting unit. ### Claim 2 (depends on 1) The device of claim 1, wherein the electromagnetic wave modulating unit comprises at least one modulation pattern. ### Claim 3 (depends on 2) The device of claim 2, wherein the at least one modulation pattern comprises a fixed modulation pattern or a random pattern. ### Claim 4 (depends on 1) The device of claim 1, further comprising a relative pattern area calculating unit configured to select a modulation area corresponding to the at least one intensity of the at least one modulated electromagnetic wave detected by the electromagnetic wave intensity detecting unit. ### Claim 5 (depends on 1) The device of claim 1, wherein the generation unit is configured to generate the at least the phase image or the amplitude image by performing a sparsity-constrained operation on the at least one intensity of the at least one modulated electromagnetic wave based on a sparsity of the object. ### Claim 6 (depends on 1) The device of claim 1, wherein the electromagnetic wave intensity detecting unit comprises a single-pixel detector. ### Claim 7 A method for imaging an object comprising: (a) emitting at least one electromagnetic wave, having at least partial coherence, towards a flow path to cause at least one optical interaction between the at least one electromagnetic wave and the object; (b) directing an electromagnetic wave modulating unit to modulate one or both of a phase or an amplitude of the at least one electromagnetic wave following the at least one optical interaction to produce at least one modulated electromagnetic wave, wherein the electromagnetic wave modulating unit comprises a plurality of areas configured to modulate the at least one electromagnetic wave differently; (c) detecting at least one intensity of the at least one modulated electromagnetic wave; and (d) generating at least a phase image or an amplitude image of the object based at least in part on the at least one intensity of the at least one modulated electromagnetic wave detected in (c). ### Claim 8 (depends on 7) The method of claim 7, wherein the electromagnetic wave modulating unit comprises at least one modulation pattern. ### Claim 9 (depends on 8) The method of claim 8, wherein the at least one modulation pattern comprises a fixed modulation pattern or a random pattern. ### Claim 10 (depends on 7) The method of claim 7, further comprising selecting a modulation area corresponding to the at least one intensity of the at least one modulated electromagnetic wave detected in (c). ### Claim 11 (depends on 7) The method of claim 7, wherein generating the at least the phase image or the amplitude image comprises performing a sparsity-constrained operation on the at least one intensity of the at least one modulated electromagnetic wave based on a sparsity of the object. ### Claim 12 (depends on 8) The method of claim 8, wherein the at least one modulation pattern is selected based on the object. ### Claim 13 (depends on 7) The method of claim 7, wherein the at least one intensity of the at least one modulated electromagnetic wave is detected using a single-pixel detector. ### Claim 14 A non-transitory computer-readable medium comprising machine-executable code that, upon execution by one or more computer processors, implements a method for imaging an object flowing through a flow path: (a) emitting at least one electromagnetic wave, having at least partial coherence, towards a flow path to cause at least one optical interaction between the at least one electromagnetic wave and the object; (b) directing an electromagnetic wave modulating unit to modulate one or both of a phase or an amplitude of the at least one electromagnetic wave following the at least one optical interaction to produce at least one modulated electromagnetic wave, wherein the electromagnetic wave modulating unit comprises a plurality of areas configured to modulate the at least one electromagnetic wave differently; (c) detecting at least one intensity of the at least one modulated electromagnetic wave; and (d) generating at least a phase image or an amplitude image of the object based at least in part on the at least one intensity of the at least one modulated electromagnetic wave detected in (c). ### Claim 15 (depends on 14) The non-transitory computer-readable medium of claim 14, wherein the electromagnetic wave modulating unit comprises at least one modulation pattern. ### Claim 16 (depends on 15) The non-transitory computer-readable medium of claim 15, wherein the at least one modulation pattern comprises a fixed modulation pattern or a random pattern. ### Claim 17 (depends on 14) The non-transitory computer-readable medium of claim 14, wherein the method further comprises selecting a modulation area corresponding to the at least one intensity of the at least one modulated electromagnetic wave detected in (c). ### Claim 18 (depends on 14) The non-transitory computer-readable medium of claim 14, wherein generating the at least the phase image or the amplitude image comprises performing a sparsity-constrained operation on the at least one intensity of the at least one modulated electromagnetic wave based on a sparsity of the object. ### Claim 19 (depends on 14) The non-transitory computer-readable medium of claim 14, wherein the at least one intensity of the at least one modulated electromagnetic wave is detected using a single-pixel detector. ### Claim 20 (depends on 15) The non-transitory computer-readable medium of claim 15, wherein the at least one modulation pattern is selected based on the object.