# US 11,906,563 B2 · Electric potential measuring device and method for manufacturing electric potential measuring device > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,906,563 B2 - Document ID: US11906563B2 - Status: Granted - Kind: B2 - Application number: 17/250,183 - Filed: 2019-06-12 - Published: 2021-06-10 - Granted: 2024-02-20 - Earliest claimed: 2018-06-19 - Assignees: Sony Semiconductor Solutions Corporation - Applicants: Sony Semiconductor Solutions Corporation - Inventors: Naohiko Kimizuka - Web page: https://patentgrep.com/patent/US11906563B2 ## CPC - G01N 27/3277: {being a redox reaction, e.g. detection by cyclic voltammetry (voltammetry per se G01N27/42, G01N27/48)} - G01R 29/12: Measuring electrostatic fields {or voltage-potential} - C12M 1/00: Apparatus for enzymology or microbiology - C12M 1/34: Measuring or testing with condition measuring or sensing means, e.g. colony counters - G01N 27/301: {Reference electrodes} - G01N 27/416: Systems (G01N27/27 takes precedence) - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - G01R: MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES (indicating correct tuning of resonant circuits H03J3/12) - C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS} ## Abstract To provide an electric potential measuring device that can further improve evaluation quality. Provided is an electric potential measuring device including a plurality of readout electrodes arranged in an array form and each configured to detect an electric potential of an action potential generation point generated by an action of a cell, an insulating member, a reference electrode configured to detect a reference potential, and an amplification section configured to obtain a potential difference between a detected electric potential based on the readout electrode and a detected electric potential based on the reference electrode, in which the readout electrode has a covered region where the insulating member is stacked on the readout electrode and an opened region where the insulating member is not stacked on the readout electrode, and the readout electrode has, in the opened region, at least one high portion with high height and/or at least one low portion with low height, with a stacking surface of the readout electrode with the insulating member as a standard. ## Claims ### Claim 1 An electric potential measuring device comprising: a plurality of readout electrodes in an array form, wherein each readout electrode of the plurality of readout electrodes is configured to detect an electric potential of an action potential generation point, and the electric potential is generated by an action of a cell; an insulating member; a reference electrode configured to detect a reference potential, wherein the reference electrode is within the array of the plurality of readout electrodes; and an amplification section configured to obtain a potential difference between the detected electric potential and the detected reference potential, wherein each readout electrode has a covered region and an opened region, the insulating member is present on the covered region of each readout electrode, the insulating member is absent on the opened region of each readout electrode, and each readout electrode has, in the opened region, at least one high portion with a first height and at least one low portion with a second height, the first height is higher than the second height with a stacking surface of a corresponding readout electrode with the insulating member as a standard. ### Claim 2 (depends on 1) The electric potential measuring device according to claim 1, wherein a shape of a surface of the opened region is uneven. ### Claim 3 An electric potential measuring device comprising: a plurality of readout electrodes in an array form, wherein each read electrode of the plurality of readout electrodes is configured to detect an electric potential of an action potential generation point, and the electric potential is generated by an action of a cell; an insulating member; a metal member; a reference electrode configured to detect a reference potential, wherein the reference electrode is within the array of the plurality of readout electrodes; and an amplification section configured to obtain a potential difference between the detected electric potential and the detected reference potential, wherein each readout electrode has a covered region and an opened region, the metal member and the insulating member are present on the covered region of each readout electrode, the metal member and the insulating member are absent on the opened region of each readout electrode, and each readout electrode has, in the opened region, at least one high portion with a first height and at least one low portion with a second height, the first height is higher than second height with a stacking surface of a corresponding readout electrode with the insulating member as a standard. ### Claim 4 (depends on 3) The electric potential measuring device according to claim 3, wherein each readout electrode has, in the opened region, at least one portion with higher height with respect to a stacking surface of the metal member with the insulating member as a standard. ### Claim 5 (depends on 3) The electric potential measuring device according to claim 3, wherein a shape of a surface of the opened region is uneven. ### Claim 6 A method for manufacturing an electric potential measuring device, the method comprising: stacking an insulating member on a readout electrode; forming, on the readout electrode, an opened region where the insulating member is not stacked; performing an electrochemical oxidation-reduction cycle on the readout electrode having the opened region; and forming, on a surface of the opened region, at least one high portion with a first height and at least one low portion with a second height, wherein the first height is higher than the second height with a stacking surface of the readout electrode with the insulating member as a standard. ### Claim 7 (depends on 6) The method for manufacturing the electric potential measuring device according to claim 6, the method further comprising removing a substance attached to a surface of the opened region. ### Claim 8 (depends on 6) The method for manufacturing the electric potential measuring device according to claim 6, the method further comprising forming an uneven shape on a surface of the opened region. ### Claim 9 A method for manufacturing an electric potential measuring device, the method comprising: stacking a metal member and an insulating member in this order on a readout electrode; forming, on the readout electrode, an opened region where the metal member and the insulating member are not stacked; performing an electrochemical oxidation-reduction cycle on the readout electrode having the opened region; and forming, on a surface of the opened region, at least one high portion with a first height and at least one low portion with a second height, wherein the first height is higher than the second height with a stacking surface of the readout electrode with the metal member as a standard. ### Claim 10 (depends on 9) The method for manufacturing the electric potential measuring device according to claim 9, the method further comprising removing a substance attached to a surface of the opened region. ### Claim 11 (depends on 9) The method for manufacturing the electric potential measuring device according to claim 9, the method further comprising forming, on a surface of the opened region, at least one portion with a higher height with respect to a stacking surface of the metal member with the insulating member as a standard. ### Claim 12 (depends on 9) The method for manufacturing the electric potential measuring device according to claim 9, the method further comprising forming an uneven shape on a surface of the opened region.