# US 11,906,529 B1 · Methods for treatment and diagnosis of endometriosis

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- Number: US 11,906,529 B1
- Document ID: US11906529B1
- Status: Granted
- Kind: B1
- Application number: 16/397,825
- Filed: 2019-04-29
- Granted: 2024-02-20
- Earliest claimed: 2018-04-27
- Assignees: Marshall University Research Corporation
- Applicants: Marshall University Research Corporation
- Inventors: Nalini Santanam; Kristeena Ray Wright; Sarah Brunty
- Web page: https://patentgrep.com/patent/US11906529B1

## CPC

- G01N 33/6893: {related to diseases not provided for elsewhere}
- A61K 31/496: Non-condensed piperazines containing further heterocyclic rings, e.g. rifampin, thiothixene or sparfloxacin
- A61K 31/5377: not condensed and containing further heterocyclic rings, e.g. timolol
- C12Q 1/6883: for diseases caused by alterations of genetic material
- C12Q 2600/106: Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism
- C12Q 2600/178: miRNA, siRNA or ncRNA
- G01N 2333/4703: Regulators; Modulating activity
- G01N 2800/364: Endometriosis, i.e. non-malignant disorder in which functioning endometrial tissue is present outside the uterine cavity
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

Methods and assays for diagnosis or prognosis of endometriosis include comparing an expression level or activity of miR-155 and JARID2 in a sample to detect a measurable difference. Therapeutic methods for treating endometriosis in a subject comprise identifying a subject as having a decreased expression level and/or activity of JARID2 in a sample obtained from the subject; and administering an agent that inhibits an activity of an miRNA that targets JARID2 or an agent that inhibits EZH2. Methods for screening for a compound useful for treating endometriosis are also provided and include contacting a cell with an effective amount of a test compound, and detecting whether the expression level or activity level of JARID2 or miR-155 in the cell is altered in the presence of the test compound.

## Claims

### Claim 1

A method for screening for a compound useful for treating endometriosis, comprising:
contacting an endometrial cell with an effective amount of a test compound;
detecting an activity level of JARID2 and miR-155 in the endometrial cell in the presence of the test compound; and
comparing the activity level of JARID2 and miR-155 in the presence of the test compound to a control activity level of JARID2 and miR-155 in a non-contacted endometrial cell, wherein the test compound is identified as a compound useful for the treatment of endometriosis if there is a measurable decrease in the activity level of JARID2 and an increase in the activity level of miR-155 in the contacted endometrial cell as compared to the control activity level of JARID2 and miR-155.

### Claim 2 (depends on 1)

The method of claim 1, further comprising determining an activity level of EZH2 and/or H3k27me3 in the contacted endometrial cell in the presence of the test compound.

### Claim 3 (depends on 1)

The method of claim 1, wherein the contacted endometrial cell is obtained from a subject.

### Claim 4 (depends on 3)

The method of claim 3, wherein the subject is human.

### Claim 5 (depends on 3)

The method of claim 3, wherein the subject has endometriosis.

### Claim 6 (depends on 5)

The method of claim 5, wherein the endometriosis is eutopic endometriosis or ectopic endometriosis.

### Claim 7 (depends on 5)

The method of claim 5, wherein the subject has pain associated with the endometriosis.