# US 11,906,523 B2 · Signaling conjugates and methods of use

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- Number: US 11,906,523 B2
- Document ID: US11906523B2
- Status: Granted
- Kind: B2
- Application number: 16/856,589
- Filed: 2020-04-23
- Published: 2020-08-06
- Granted: 2024-02-20
- Earliest claimed: 2012-03-27
- Assignees: Ventana Medical Systems, Inc.
- Applicants: Ventana Medical Systems, Inc.
- Inventors: Nelson Alexander; William Day; Jerome W. Kosmeder, II; Mark Lefever; Larry Morrison; Anne M. Pedata; Stacey Stanislaw
- Web page: https://patentgrep.com/patent/US11906523B2

## CPC

- G01N 33/581: {with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)}
- C12Q 1/68: involving nucleic acids
- C12Q 1/682: Signal amplification
- G01N 33/53: Immunoassay; Biospecific binding assay; Materials therefor
- G01N 33/542: with steric inhibition or signal modification, e.g. fluorescent quenching
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

Disclosed herein are embodiments of a signaling conjugate, embodiments of a method of using the signaling conjugates, and embodiments of a kit comprising the signaling conjugate. The disclosed signaling conjugate comprises a latent reactive moiety and a chromogenic moiety that may further comprise a linker suitable for coupling the latent reactive moiety to the chromogenic moiety. The signaling conjugate may be used to detect one or more targets in a biological sample and are capable of being covalently deposited directly on or proximally to the target. Particular disclosed embodiments of the method of using the signaling conjugate comprise multiplexing methods.

## Claims

### Claim 1

A stained biological sample, comprising:
(a) a binding complex comprising a target, a detection probe specifically bound to the target, and an enzyme covalently linked to the detection probe, wherein the enzyme comprises a peroxidase, and wherein the target comprises a polypeptide, a nucleic acid, or a tyrosine-containing moiety;
(b) a deposited complex comprising a signaling conjugate comprising a phenolic moiety covalently linked to a chromogenic moiety, wherein the phenolic moiety of the signaling conjugate is covalently bound to: (1) a location on the binding complex proximate to or directly on the enzyme; or (2) a location on the binding complex proximate to or directly on the target;
wherein the stained biological sample is immobilized on a solid support, and wherein the chromogenic moiety of the deposited complex is capable of absorbing an incident light comprising a visible light and is present in an amount sufficient to produce a signal in a visible, light spectrum which can be detected using a bright field microscopy, wherein said signal is used for a specific detection of the target in the stained biological sample when said chromogenic moiety of the deposited complex is exposed to the incident light comprising the visible light, and wherein said signal is a spectral absorbance of the incident light generated by the chromogenic moiety of the deposited complex.

### Claim 2 (depends on 1)

The stained biological sample according to claim 1, wherein the signaling conjugate further comprises a linker joining the chromogenic moiety and the phenolic moiety.

### Claim 3 (depends on 2)

The stained biological sample according to claim 2, wherein the linker comprises polyethylene glycol.

### Claim 4 (depends on 3)

The stained biological sample according to claim 3, wherein the phenolic moiety of the signaling conjugate is a single phenolic moiety.

### Claim 5 (depends on 4)

The stained biological sample according to claim 4, wherein the chromogenic moiety comprises rhodamine, a rhodamine derivative, tetramethylrhodamine (TMR, TAMRA), diarylrhodamine derivatives, QSY 7, QSY 9, or QSY 21.

### Claim 6 (depends on 3)

The stained biological sample according to claim 3, wherein the chromogenic moiety comprises rhodamine, a rhodamine derivative, tetramethylrhodamine (TMR, TAMRA), diarylrhodamine derivatives, QSY 7, QSY 9, or QSY 21.

### Claim 7 (depends on 2)

The stained biological sample according to claim 2, wherein the chromogenic moiety comprises rhodamine, a rhodamine derivative, tetramethylrhodamine (TMR, TAMRA), diarylrhodamine derivatives, QSY 7, QSY 9, or QSY 21.

### Claim 8 (depends on 1)

The stained biological sample according to claim 1, wherein the chromogenic moiety comprises rhodamine, a rhodamine derivative, tetramethylrhodamine (TMR, TAMRA), diarylrhodamine derivatives, QSY 7, QSY 9, or QSY 21.

### Claim 9 (depends on 1)

The stained biological sample according to claim 1, wherein the chromogenic moiety comprises a triarylmethane chromogen.

### Claim 10 (depends on 1)

The stained biological sample according to claim 1, wherein the chromogenic moiety is selected from the group consisting of tartrazine, 7-diethylaminocoumarin -3-carboxylic acid, DABSYL, fluorescein, a fluorescein derivative, rhodamine, a rhodamine derivative, Rhodamine Green, Rhodamine Green carboxylic acid, an eosin derivative, eosin, 6-carboxy-2′,4,7,7′-tetrachlorofluorescein (TET), carboxyrhodamine 6G, tetramethylrhodamine, carboxytetramethylrhodamine (TMR), QSY 9, sulforhodamine B, a diarylrhodamine derivative, QSY 7, Texas Red, Fast Green FCF, Malachite Green, QSY 21, and Victoria Blue, wherein said chromogenic moiety is covalently linked to said phenolic moiety by:
(i) an amine-reactive group which is an isothiocyanate, an isocyanate, an acyl azide, an NHS ester, an acid chloride, a sulfonyl chloride, a succinimidyl ester, an aldehyde, a glyoxal, an epoxide, an oxirane, a carbonate, an arylating agent, an imidoester, a carbodiimide, or an anhydride;
(ii) a thiol-reactive group which is a haloacetyl halide, an alkyl halide, a maleimide, an aziridine, an acryloyl derivative, an arylating agent, a thiol-disulfide exchange reagent, a pyridyl disulfide, a TNB-thiol, or a disulfide reductant;
(iii) a carboxylate-reactive group which is a diazoalkane, a diazoacetyl compound, a carbonyldiimidazole compound, or a carbodiimide;
(iv) a hydroxyl-reactive group which is an epoxide, a carbonyldiimidazole, an N,N′-disuccinimidyl carbonate, an N-hydroxysuccinimidyl chloroformate, a periodate oxidizing compound, an alkyl halide, or an isocyanate;
(v) an aldehyde and ketone-reactive group which is a hydrazine, a Schiff base, a reductive amination product, or a Mannich condensation product;
(vi) an active hydrogen-reactive group which is a diazonium derivative, a Mannich condensation product, or an iodination reaction product; or
(vii) a photoreactive group which is an aryl azide, a halogenated aryl azide, a benzophenone, a diazo compound, or a diazirine derivative.

### Claim 11 (depends on 1)

The stained biological sample according to claim 1, wherein the peroxidase is horseradish peroxidase.

### Claim 12 (depends on 1)

The stained biological sample according to claim 1, wherein said spectral absorbance of the incident light generated by the chromogenic moiety of the deposited complex includes a non-uniform absorbance.

### Claim 13

A stained biological sample, comprising:
(a) a binding complex comprising a target antigen, a detection probe specifically bound to the target antigen, and an enzyme covalently linked to the detection probe, wherein the enzyme comprises a peroxidase, and the detection probe comprises an antibody specific for the target antigen;
(b) a deposited complex comprising a signaling conjugate comprising a phenolic moiety covalently linked to a chromogenic moiety by a linker joining the phenolic moiety and the chromogenic moiety, wherein the phenolic moiety of the signaling conjugate is covalently bound to: (1) a location on the binding complex proximate to or directly on the enzyme; or (2) a location on the binding complex proximate to or directly on the target antigen;
wherein the stained biological sample is immobilized on a solid support, and the chromogenic moiety of the deposited complex is capable of absorbing an incident light comprising a visible light and is present in an amount sufficient to produce a signal in a visible, light spectrum which can be detected using a bright field microscopy, wherein said signal is used for a specific detection of the target antigen in the stained biological sample when said chromogenic moiety of the deposited complex is exposed to the incident light comprising the visible light, and wherein the signal is a spectral absorbance of the incident light generated by the chromogenic moiety of the deposited complex.

### Claim 14 (depends on 13)

The stained biological sample according to claim 13, wherein the chromogenic moiety comprises rhodamine, a rhodamine derivative, tetramethylrhodamine (TMR, TAMRA), diarylrhodamine derivatives, QSY 7, QSY 9, or QSY 21.

### Claim 15 (depends on 13)

The stained biological sample according to claim 13, wherein said spectral absorbance of the incident light generated by the chromogenic moiety of the deposited complex includes a non-uniform absorbance.

### Claim 16

A stained biological sample, comprising:
(a) a binding complex comprising a target antigen, a detection probe specifically bound to the target antigen, and an enzyme covalently linked to the detection probe, wherein the enzyme comprises a peroxidase, and the detection probe comprises an antibody specific for the target antigen;
(b) a deposited complex comprising a signaling conjugate comprising a phenolic moiety covalently linked to a chromogenic moiety comprising rhodamine or a rhodamine derivative, wherein the phenolic moiety of the signaling conjugate is covalently bound to: (1) a location on the binding complex proximate to or directly on the enzyme; or (2) a location on the binding complex proximate to or directly on the target antigen;
wherein the stained biological sample is immobilized on a solid support, and wherein the chromogenic moiety of the deposited complex is capable of absorbing an incident light comprising a visible light and is present in an amount sufficient to produce a signal in a visible, light spectrum which can be detected using a bright field microscopy, wherein said signal is used for a specific detection of the target antigen in the stained biological sample when said chromogenic moiety of the deposited complex is exposed to the incident light comprising the visible light, and wherein the signal is a spectral absorbance of the incident light generated by the chromogenic moiety of the deposited complex.

### Claim 17 (depends on 16)

The stained biological sample according to claim 16, wherein the phenolic moiety of the signaling conjugate is a single phenolic moiety.

### Claim 18 (depends on 17)

The stained biological sample according to claim 17, wherein the signaling conjugate further comprises a linker joining the phenolic moiety and the chromogenic moiety.

### Claim 19 (depends on 16)

The stained biological sample according to claim 16, wherein said spectral absorbance of the incident light generated by the chromogenic moiety of the deposited complex includes a non-uniform absorbance.

### Claim 20

A stained biological sample, comprising:
(a) a binding complex comprising a target antigen, a detection probe specifically bound to the target antigen, and an enzyme covalently linked to the detection probe, wherein the enzyme comprises a peroxidase, and the detection probe comprises an antibody specific for the target antigen;
(b) a deposited complex comprising a signaling conjugate comprising only a single phenolic moiety covalently linked to a chromogenic moiety comprising rhodamine or a rhodamine derivative by a linker joining the phenolic moiety and the chromogenic moiety, wherein the linker comprises polyethylene glycol, wherein the phenolic moiety of the signaling conjugate is covalently bound to: (1) a location on the binding complex proximate to or directly on the enzyme; or (2) a location on the binding complex proximate to or directly on the target antigen;
wherein the stained biological sample is immobilized on a solid support, and wherein the chromogenic moiety of the deposited complex is capable of absorbing an incident light comprising a visible light and is present in an amount sufficient to produce a colored signal in a visible light spectrum which can be detected using a bright field microscopy, wherein said colored signal is used for a specific detection of the target antigen in the stained biological sample when said chromogenic moiety of the deposited complex is exposed to the incident light comprising the visible light, and wherein the colored signal is a spectral absorbance of the incident light generated by the chromogenic moiety of the deposited complex.

### Claim 21 (depends on 20)

The stained biological sample according to claim 20, wherein said spectral absorbance of the incident light generated by the chromogenic moiety of the deposited complex includes a non-uniform absorbance.