Multiplexed lateral flow assay device

A multiplexed lateral flow device, comprising:

• an impermeable internal reservoir having a body with an upper surface, a lower surface, and an opening in the body to receive a sample deposition;
• a fluid distributor pad arranged in fluid communication with a lower surface of the internal reservoir, the fluid distributor pad comprising a paper-based microfluidic element having a pattern of a hydrophobic material to distribute the sample deposition substantially equally among a plurality of flow paths;
• two or more lateral flow assays having a plurality of flow lines arranged in fluid communication with the plurality of flow paths of the fluid distributor pad;
• an impermeable top cover having a first window arranged over the opening of the internal reservoir, and a second window arranged over a test result of the lateral flow assays;
• an impermeable bottom cover opposing the impermeable top cover;
• and
• an impermeable housing element arranged between the impermeable top cover and the impermeable bottom cover;
• wherein the reservoir, the fluid distributor pad and the two or more lateral flow assays are arranged within the housing element, and
• wherein the housing element includes a spacer element configured to provide a gap between the two or more lateral flow assays and impermeable top cover.