# US 11,906,511 B2 · Methods and compositions for treating diseases associated with exhausted T cells

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- Number: US 11,906,511 B2
- Document ID: US11906511B2
- Status: Granted
- Kind: B2
- Application number: 16/759,676
- Filed: 2018-10-26
- Published: 2021-02-04
- Granted: 2024-02-20
- Earliest claimed: 2017-10-27
- Assignees: The Trustees of the University of Pennsylvania
- Applicants: The Trustees of the University of Pennsylvania
- Inventors: E. John Wherry; Bertram Bengsch; Omar Khan; Jennifer Wu; Josephine Giles
- Web page: https://patentgrep.com/patent/US11906511B2

## CPC

- C12Q 1/6883: for diseases caused by alterations of genetic material
- G01N 33/505: {involving T-cells}
- C12Q 1/686: Polymerase chain reaction [PCR]
- C12Q 1/6869: Methods for sequencing
- C12Q 1/6886: for cancer (immunoassay for cancer G01N33/575)
- G01N 33/5091: {for testing the pathological state of an organism}
- G01N 33/56972: {White blood cells}
- C12Q 2600/118: Prognosis of disease development
- C12Q 2600/154: Methylation markers
- C12Q 2600/158: Expression markers
- G01N 2800/52: Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

The present invention provides compositions and methods for detecting exhausted T cells in a subject. The present invention also provides methods for treating a subject having a disease characterized by the presence of exhausted T cells or certain subpopulations thereof.

## Claims

### Claim 1

A method for detecting exhausted T cells in a subject, wherein the method comprises
detecting an OCR footprint in T cells from the subject, wherein the OCR footprint is correlated with exhausted T cells, and wherein said OCR footprint in said T cells from the subject is determined by a method comprising conducting quantitative PCR (qPCR) on a chromatin library from said population of T cells from the subject, and conducting high-throughput array-based testing on amplification products of said qPCR.

### Claim 2

A method for detecting exhausted T cells in a subject, wherein the method comprises detecting an OCR footprint in T cells from the subject, wherein the OCR footprint is correlated with exhausted T cells, and wherein said OCR footprint in said T cells from the subject is determined by a method comprising conducting quantitative PCR (qPCR) on a chromatin library from said population of T cells from the subject, and conducting multi-locus qPCR testing.