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US 11,905,566 B2Granted
Methods of detecting listeria from an environmental sample
AssigneeNeogen Corporation
InventorsJohn Nelson, Preetha Biswas, Lei Zhang, Mark A. Mozola, Lisa Pinkava, David Brookman, Edan Robert Hosking
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Abstract
The present invention relates to the identification of microorganisms from an environmental sample, and in particular to the rapid identification of Listeria spp. The methods and kits described herein provide a method of detecting Listeria spp. without the need for an enrichment step.
Claims5
A composition comprising a lysis reagent solution comprised of sodium sulfate, magnesium sulfate, poly(ethylene oxide)octylphenyl ether, potassium phosphate, lysozyme, and proteinase K;
- and
- an isothermal nucleic acid amplification assay comprised of (i) a polymerase, (ii) a first template nucleic acid that hybridizes to a first strand of a target nucleotide sequence, and (iii) a second template nucleic acid that hybridizes to a complement of the first strand of the target nucleotide sequence and (iv) a nicking enzyme,
- wherein the composition does not include enrichment broth.
A composition comprising:
- a lysis reagent solution comprised of sodium sulfate, magnesium sulfate, poly(ethylene oxide)octylphenyl ether, potassium phosphate, lysozyme, and proteinase K;
- and
- a sample of Listeria,
- wherein the composition does not include enrichment broth.
1 more element
Dependencies are inferred from the claim text.