System and method for producing interleukin receptor antagonist (IRA)
AssigneeEstar Technologies Ltd
InventorAaron Esteron
Life of this patent
Estimating the term…
Abstract
A system and method for producing high levels of autologous IL-1RA cytokine, includes a blood collection vessel, a cover, a portion of separation gel, an anticoagulant portion, a plasma collection syringe comprising a sharp needle, a buffy coat collection syringe and an incubation tube with cover. The blood collection vessel is adapted such that when containing whole blood and being centrifuged, after treatment yields separation fractions comprising, a first fraction of RBCs sediment, a second fraction of said gel, a third fraction comprising WBCs, platelets and a fourth fraction of plasma solution.
Claims2
A method for producing of autologous IL-1 receptor antagonist (IL1-RA) cytokine in a closed vessel tube system, the method comprising the steps of:
- a. obtaining a vessel tube system for producing autologous IL-1RA cytokine comprising:
- (i) an incubation vessel comprising washed and pretreated borosilicate glass beads in a size of about 0.5-5 mm for collection of a blood sample;
- and
- (ii) a vessel cover;
- b. filling said incubation vessel with whole blood sample;
- c. incubating said incubation vessel with said whole blood sample for 30 minutes to 24 hours at 25-37° C.;
- d. centrifuging said vessel for about 10-30 minutes;
- and
- filtering an enriched plasma fraction obtained from said vessel after centrifuging with a filter having a pore size of 0.2-50 μm such that a concentration of said autologous IL-1RA cytokine relative to said whole blood sample is increased.
Dependencies are inferred from the claim text.