North American porcine reproductive and respiratory syndrome (PRRS) virus and uses thereof
AssigneeZoetis Services LLC
InventorsJay Gregory Calvert, David Ewell Slade, Siao-Kun W. Welch
Life of this patent
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Abstract
The invention provides isolated polynucleotide molecules that comprise a DNA sequence encoding an infectious RNA sequence encoding a genetically-modified North American PRRS virus, methods to make it and related polypeptides, polynucleotides, and various components. Vaccines comprising the genetically modified virus and polynucleotides and a diagnostic kit to distinguish between naturally infected and vaccinated animals are also provided.
Claims4
A method of reducing Porcine Reproductive and Respiratory Syndrome Virus (PRRS)-induced reproductive symptoms in a sow, the method comprising administering to said sow:
- a) an isolated Porcine Reproductive and Respiratory Syndrome Virus (PRRS) wherein the protein thereof encoded by ORF1a is selected from a group consisting of those amino acid sequences that contain:
- i) one, two, three or four of:
- an amino acid N within the amino acid sequence AMANVYD (SEQ ID NO:
- 9);
- an amino acid N within the amino acid sequence IGHNAVM (SEQ ID NO:
- 12);
- an amino acid Y within the amino acid sequence CVVWYLFD (SEQ ID NO:
- 18);
- and
- an amino acid H within the amino acid sequence HGVHGKY (SEQ ID NO:
- 21);
- and
- ii) one or both of:
- an amino acid T within the amino acid sequence PSATDTS (SEQ ID NO:
- 27);
- and
- an amino acid C within the amino acid sequence APMCQDE (SEQ ID NO:
- 33);
- and
- iii) one or both of:
- an amino acid T within the amino acid sequence CAPTGMD (SEQ ID NO:
- 36);
- and
- an amino acid A within the amino acid sequence PKVAKVS (SEQ ID NO:
- 39);
- and
- iv) an amino acid N within the amino acid sequence ADFNPEK (SEQ ID NO:
- 45);
- or
- b) a plasm id capable of directly transfecting a suitable host cell and expressing a Porcine Reproductive and Respiratory Syndrome Virus (PRRS) from the suitable host cell so transfected, which plasm id comprises:
- (1) a DNA sequence encoding an infectious RNA molecule encoding the PRRS virus, and (2) a promoter capable of transcribing said infectious RNA molecule, wherein the protein encoded by ORF1a of said virus has an amino acid sequence that contains:
- (i) one, two, three, or four of
- an amino acid N within the amino acid sequence ANV (see SEQ ID NO:
- 9);
- an amino acid N within the amino acid sequence HNA (see SEQ ID NO:
- 12);
- an amino acid Y within the amino acid sequence WYL (see SEQ ID NO:
- 18);
- and
- an amino acid H within the amino acid sequence VHG (see SEQ ID NO:
- 21);
- and
- (ii) one or both of
- an amino acid T within the amino acid sequence ATD (see SEQ ID NO:
- 27);
- and
- an amino acid C within the amino acid sequence MCQ (see SEQ ID NO:
- 33);
- and
- (iii) one or both of
- an amino acid T within the amino acid sequence PTG (see SEQ ID NO:
- 36);
- and
- an amino acid A within the amino acid sequence VAK (see SEQ ID NO:
- 39);
- and
- (4) an amino acid N within the amino acid sequence FNP (see SEQ ID NO:
- 45).
Dependencies are inferred from the claim text.