Recombinant virus capable of stably expressing target proteins
AssigneeBoehringer Ingelheim Vetmedica (China) Co., Ltd.
InventorsGuanggang Ma, Sen Yuan
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Abstract
Provided is a method to generate a recombinant virus that can stably express target proteins. The recombinant virus of the present invention is useful for producing an immunogenic composition or vaccine.
Claims25
A recombinant virus comprising an expression cassette in the genome of the recombinant virus, wherein the expression cassette comprises a first polynucleotide encoding at least one polypeptide of interest, and a second polynucleotide which is an essential gene of the recombinant virus or a functional fragment thereof, and wherein the first polynucleotide is functionally linked to the second polynucleotide with the first polynucleotide being located upstream of the second polynucleotide, and wherein said second polynucleotide is the only copy of said essential gene or functional fragment thereof that is active in the recombinant virus, and wherein the first polynucleotide and the second polynucleotide are within the same open reading frame (ORF).
A recombinant virus comprising an expression cassette in the genome of the recombinant virus, wherein the expression cassette comprises a first polynucleotide encoding at least one polypeptide of interest, and a second polynucleotide which is an essential gene of the recombinant virus or a functional fragment thereof;
- wherein the first polynucleotide is functionally linked to the second polynucleotide with the first polynucleotide being located upstream of the second polynucleotide;
- wherein said second polynucleotide is the only copy of said essential gene or functional fragment there that is active in the recombinant virus;
- wherein the first polynucleotide and the second polynucleotide are within the same ORF;
- and
- wherein the recombinant virus is derived from EHV-1, the first polynucleotide comprises the P1 gene, 2A gene and 3C gene of FMDV, and the essential gene is EHV-1 ORF43 gene or EHV-1 ORF54 gene.
A recombinant virus, comprising an expression cassette in the genome of the recombinant virus, wherein the expression cassette comprises a first polynucleotide encoding at least one polypeptide of interest, and a second polynucleotide which is an essential gene of the recombinant virus or a functional fragment thereof;
- wherein the first polynucleotide is functionally linked to the second polynucleotide with the first polynucleotide being located upstream of the second polynucleotide;
- wherein said second polynucleotide is the only copy of said essential gene or functional fragment there that is active in the recombinant virus;
- wherein the first polynucleotide and the second polynucleotide are within the same ORF;
- wherein the expression cassette further comprises regulatory elements;
- and
- wherein the expression cassette comprises a P1-2A-3C-2A-ORF43 construct or a P1-2A-3C-2A-ORF54 construct.
A recombinant virus, comprising an expression cassette in the genome of the recombinant virus, wherein the expression cassette comprises a first polynucleotide encoding at least one polypeptide of interest, and a second polynucleotide which is an essential gene of the recombinant virus or a functional fragment thereof;
- wherein the first polynucleotide is functionally linked to the second polynucleotide with the first polynucleotide being located upstream of the second polynucleotide;
- wherein said second polynucleotide is the only copy of said essential gene or functional fragment there that is active in the recombinant virus;
- wherein the first polynucleotide and the second polynucleotide are within the same ORE;
- and
- wherein the expression cassette comprises a CMV-P1-2A-3C-2A-ORF43-BGH construct as shown in SEQ ID NO.:
- 8, or a CMV-P1-2A-3C-2A-ORF54-BGH construct as shown in SEQ ID NO.:
- 9.
Dependencies are inferred from the claim text.