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US 11,900,595 B2Granted
Imaging systems and methods useful for patterned structures
AssigneeSingular Genomics Systems, Inc.
InventorsFedor Trintchouk, Eli N. Glezer
Life of this patent
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Abstract
Disclosed herein, inter alia, are methods and systems of image analysis useful for identifying and/or quantifying features in patterns.
Claims16
A method of quantifying features in a repeating pattern of an object, the method comprising:
- obtaining an image of the object using a detection apparatus, wherein the image includes a repeating pattern of features having different signal levels;
- providing the image or image-related data to a computer, wherein the computer has parameter data that describe the repeating pattern of features;
- partitioning the image or the image-related data into a plurality of registration subimages on the computer;
- detecting on the computer the repeating pattern of features for each registration subimage;
- assigning an index address for each feature of the repeating pattern of features;
- quantifying a signal level of each feature;
- wherein a first camera collects an image of a first fluorescent channel and a second fluorescent channel and wherein a second camera collects an image of a third fluorescent channel and a fourth fluorescent channel, and further comprising performing a chromatic correction in order to associate feature images in the channels of a common camera.
4 more elements
A non-transitory computer-readable medium containing instructions to configure a processor to perform operations comprising:
- obtaining an image of the object using a detection apparatus, wherein the image includes a repeating pattern of features having different signal levels;
- providing the image or image-related data to a computer, wherein the computer has parameter data that describe the repeating pattern of features;
- partitioning the image or the image-related data into a plurality of registration subimages on the computer;
- detecting on the computer the repeating pattern of features for each registration subimage;
- assigning an index address for each feature of the repeating pattern of features;
- matching the index address for each feature on the computer;
- and
- quantifying a signal level of each feature;
- wherein a first camera collects an image of a first fluorescent channel and a second fluorescent channel and wherein a second camera collects an image of a third fluorescent channel and a fourth fluorescent channel, and the operations further comprising performing a chromatic correction in order to associate feature images in the channels of a common camera.
6 more elements
A system comprising:
- a processor;
- and
- a memory, wherein the processor and the memory are configured to perform operations comprising:
- obtaining an image of the object using a detection apparatus, wherein the image includes a repeating pattern of features having different signal levels;
- providing the image or image-related data to a computer, wherein the computer has parameter data that describe the repeating pattern of features;
- partitioning the image or the image-related data into a plurality of registration subimages on the computer;
- detecting on the computer the repeating pattern of features for each registration subimage;
- assigning an index address for each feature of the repeating pattern of features;
- matching the index address for each feature on the computer;
- and
- quantifying a signal level of each feature;
- wherein a first camera collects an image of a first fluorescent channel and a second fluorescent channel and wherein a second camera collects an image of a third fluorescent channel and a fourth fluorescent channel, and the operations further comprising performing a chromatic correction in order to associate feature images in the channels of a common camera.
9 more elements
Dependencies are inferred from the claim text.