Treatment and diagnosis of parkinson's disease using isolated and enriched populations of biofluid-derived extracellular vesicles

A method for treating Parkinson disease in a subject, the method comprising:

• a) contacting a biological sample from the subject with a functionalized capture surface to form a complex between the functionalized capture surface and EVs comprising the at least one cell surface marker, wherein the capture surface is functionalized with a reagent that specifically binds the at least one cell surface marker, wherein the at least one surface marker is Glutamate ionotropic receptor AMPA type subunit 2 (GluR2);
• b) separating the formed complex from an unbound portion of the biological sample to obtain a captured complex;
• c) washing the captured complex;
• d) eluting the EVs comprising the at least one surface marker from the functionalized capture surface or lysing the EVs complexed with the functionalized capture surface;
• e) extracting at least one target biomarker from the eluted or lysed EVs, wherein the at least one target biomarker is parkin (PARK2) RNA;
• f) determining the expression level of the at least one biomarker extracted in step (e);
• g) identifying the subject as having Parkinson disease by comparing the expression level of the at least one target biomarker to a corresponding pre-defined threshold;
• and;
• h) administering at least one therapy for Parkinson disease to the subject.