Detection of analytes using live cells
AssigneeThe Trustees of Columbia University in the City of New York
InventorsVirginia Wood Cornish, Nili Ostrov, Miguel Jimenez, Sonja Billerbeck
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Abstract
The present invention provides sensor cells comprising a receptor that binds to an analyte indicative of the presence of an agent, where binding of the analyte to the receptor triggers a detection event that is indicative of the presence of the agent. In certain embodiments, the detection event is appearance of a reporter detectable by the naked eye. The present invention also provides uses of such sensor cells for detecting the presence of an agent in a sample.
Claims27
A sensor fungal cell comprising a fungal non-native G-protein coupled receptor (GPCR) that binds to a peptide analyte derived from an agent, wherein the peptide analyte is a ligand for the fungal non-native GPCR, wherein binding of the peptide analyte to the fungal non-native GPCR triggers an appearance of a reporter in the fungal cell, wherein the appearance of the reporter indicates the presence of the agent, and wherein the reporter is a biosynthesized visible-light pigment.
A sensor fungal cell comprising a fungal non-native G-protein coupled receptor (GPCR) that binds to a peptide analyte derived from an agent, wherein the peptide analyte is a ligand for the fungal non-native GPCR, wherein binding of the peptide analyte to the fungal non-native GPCR triggers an appearance of a reporter in the fungal cell, wherein the appearance of the reporter indicates the presence of the agent, and wherein the non-native fungal GPCR is an engineered GPCR and comprises an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 6, 9, 12, 15, 18, 21, 52, 54, 56, 58, 74, 94, and 222.
Dependencies are inferred from the claim text.