Real-time and label free analyzer for in-vitro and in-vivo detecting the suspicious regions to cancer

A probe for non-invasively conducting an electrochemical assay in a target location of a living tissue, comprising:

• a sensing part, comprising:
• two reference electrodes comprising a first needle and a second needle, each of the first needle and the second needle comprising a hollow needle, each respective hollow needle of the first needle and the second hollow needle comprising a biocompatible electrically conductive hollow needle, a first respective end of each of the first needle and the second needle being connected to an electrochemical stimulator-analyzer device via a respective electrical connector, the stimulator-analyzer device comprising a potentiostat configured to measure cyclic voltammetry (CV) based diagrams;
• a working electrode located inside the first needle;
• and
• a counter electrode located inside the second needle,
• wherein each of the working electrode and the counter electrode comprises a biocompatible electrically conductive needle, a first end of the biocompatible electrically conductive needle being connected to the electrochemical stimulator-analyzer device via a respective electrical connector, the biocompatible electrically conductive needle comprising a needle tip coated with a bilayer coating, the bilayer comprising:
• a layer of silver (Ag) paste coated on the needle tip;
• and
• a layer of carboxyl-functionalized vertically aligned multi-walled carbon nanotubes (carboxyl VAMWCNTs) electrostatically grasped and adhered onto the layer of Ag paste;
• and
• a holding member, comprising:
• an insertion part with two ends, the sensing part attached to a first end of the insertion part, the insertion part configured to facilitate insertion of the sensing part into the target location;
• and
• a button at a second end of the insertion part, the button coupled with the working electrode and the counter electrode, the button being movable inside the insertion part, the working electrode and the counter electrode being releasable outside from a respective second end of the first needle and the second needle into the target location before conducting the electrochemical assay when the button being pressed, the working electrode and the counter electrode being retractable inside the two reference electrodes through the two respective second ends of the first needle and the second needle from the target location after conducting the electrochemical assay when the button is pressed again,
• wherein a used sensing part is replaceable with a fresh sensing part by the used sensing part being configured to detach from a first end of the holding member and the fresh sensing part being configured to attach to the first end of the holding member in lieu of the used sensing part.