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US 11,898,952 B2Granted
Microparticle measuring apparatus and microparticle measuring method
AssigneeSony Corporation
InventorYasunobu Kato
Life of this patent
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Abstract
Provided is a microparticle measuring apparatus including a plurality of light detection sections that detects, at different positions, optical information emitted from microparticles flowing through a flow channel. The microparticle measuring apparatus further includes a detection timing control section that controls a detection timing of each light detection section, on the basis of a trigger signal detected at a first reference channel provided in a first light detection section, and an optical signal detected at a second reference channel provided in a second light detection section that detects optical information emitted from the microparticles, at a position different from a position of the first light detection section.
Claims17
A microparticle measuring apparatus, comprising:
- a liquid sample reservoir connected to a liquid sample flow channel, wherein the liquid sample reservoir is configured to store a liquid sample including microparticles;
- a liquid sheath reservoir connected to a liquid sheath flow channel, wherein the liquid sample flow channel and the liquid sheath flow channel are connected to a flow channel;
- a sorted liquid reservoir connected to a sorting flow channel;
- a waste liquid reservoir connected to a discarding flow channel, wherein the sorting flow channel and the discarding flow channel are connected to the flow channel;
- a plurality of light detectors configured to detect, at different positions, optical information emitted from the microparticles that flow through the flow channel, wherein the plurality of light detectors includes:
- a first light detector that includes a first plurality of channels, and
- a second light detector that includes a second plurality of channels,
- the first light detector including the first plurality of channels is at a first position on the flow channel, and
- the second light detector including the second plurality of channels is at a second position on the flow channel different from the first position;
- and
- circuitry configured to:
- control a detection timing of each light detector of the plurality of light detectors based on:
- a trigger signal detected at a first reference channel of the first plurality of channels of the first light detector, and
- an optical signal detected at a second reference channel of the second plurality of channels of the second light detector;
- set a light detection process period of the first reference channel of the first plurality of channels of the first light detector based on a time period in which the trigger signal detected at the first reference channel exceeds a threshold value;
- add a first time period and a second time period to the set light detection process period, wherein
- the first time period is added prior to the set light detection process period, and
- the second time period is added subsequent to the set light detection process period;
- and
- control a detection process period of the second light detector based on the set light detection process period of the first reference channel that includes the first time period and the second time period.
17 more elements
A microparticle measuring method, comprising:
- in a microparticle measuring apparatus that includes a plurality of light detectors, a liquid sample reservoir connected to a liquid sample flow channel, a liquid sheath reservoir connected to a liquid sheath flow channel, a sorted liquid reservoir connected to a sorting flow channel, and a waste liquid reservoir connected to a discarding flow channel:
- detecting, by the plurality of light detectors, at different positions, optical information emitted from microparticles flowing through a flow channel, wherein
- the liquid sample flow channel, the liquid sheath flow channel, the sorting flow channel, and the discarding flow channel are connected to the flow channel,
- the liquid sample reservoir is configured to store a liquid sample that includes the microparticles,
- the plurality of light detectors includes:
- a first light detector that includes a first plurality of channels, and
- a second light detector that includes a second plurality of channels, the first light detector including the first plurality of channels is at a first position on the flow channel, and
- the second light detector including the second plurality of channels is at a second position on the flow channel different from the first position;
- and
- controlling a detection timing of each light detector of the plurality of light detectors based on:
- a trigger signal detected at a first reference channel of the first plurality of channels of the first light detector, and
- an optical signal detected at a second reference channel of the second plurality of channels of the second light detector;
- setting a light detection process period of the first reference channel of the first plurality of channels of the first light detector based on a time period in which the trigger signal detected at the first reference channel exceeds a threshold value;
- adding a first time period and a second time period to the set light detection process period, wherein
- the first time period is added prior to the set light detection process period, and
- the second time period is added subsequent to the set light detection process period;
- and
- controlling a detection process period of the second light detector based on the set light detection process period of the first reference channel that includes the first time period and the second time period.
15 more elements
Dependencies are inferred from the claim text.