# US 11,898,210 B2 · Tools for assessing FimH blockers therapeutic efficiency

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- Number: US 11,898,210 B2
- Document ID: US11898210B2
- Status: Granted
- Kind: B2
- Application number: 16/755,829
- Filed: 2018-10-16
- Published: 2020-09-03
- Granted: 2024-02-13
- Earliest claimed: 2017-10-16
- Assignees: Enterome S.A.
- Applicants: Enterome
- Inventors: Alessandra Cervino; Christophe Bonny; Jonathan Plassais
- Web page: https://patentgrep.com/patent/US11898210B2

## CPC

- C12Q 1/689: for bacteria
- A61K 31/7064: containing condensed or non-condensed pyrimidines
- C12Q 2600/106: Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

The present invention relates to an in vitro method for identifying subjects hosting high amounts of Fim H expressing proteobacteria in their gut, said method comprising the step of detecting the expression of the fimH gene in a stool sample of said subjects.

## Claims

### Claim 1

A method for treating a human subject suffering from Crohn Disease (CD), said method comprising the steps of:
a) isolating a nucleotide fraction of a stool sample from said subject,
b) detecting expression of the fimH gene in said nucleotide fraction by measuring the switch of the Escherichia coli fim operon transcription from OFF to ON in a nucleotide fraction of said stool sample to calculate an ON/(ON+OFF) ratio of the fimH gene, and comparing the ON/(ON+OFF) ratio with a reference value of 15%,
c) administering a mannose derivative FimH blocker to subjects whose nucleotide fraction has an ON/(ON+OFF) ratio higher than said reference value of 15%.

### Claim 2 (depends on 1)

The method of claim 1, wherein said detecting step comprises measuring the presence of the ON region of SEQ ID NO:14 of the fim operon transcription in said stool sample.

### Claim 3 (depends on 1)

The method of claim 1, wherein said detecting step comprises measuring the presence of the ON region of SEQ ID NO:14 of the fim operon transcription in said stool sample by using the primer pair of SEQ ID NO:5&7.

### Claim 4 (depends on 1)

The method of claim 1, wherein said detecting step comprises measuring the presence of the OFF region of SEQ ID NO:13 of the fim operon transcription in said stool sample.

### Claim 5 (depends on 1)

The method of claim 1, wherein said detecting step comprises measuring the presence of the OFF region of SEQ ID NO:13 of the fim operon transcription in said stool sample, by using the primer pair of SEQ ID NO:5&8.

### Claim 6 (depends on 1)

The method of claim 1, wherein said mannose derivative FimH blocker has the formula:

### Claim 7 (depends on 1)

The method of claim 1, wherein the mannose derivative FimH blocker is selected from Heptylmannose, Thiazolylmannosides, and ZFH-04269.

### Claim 8 (depends on 1)

The method of claim 1, wherein the ON/(ON+OFF) ratio for the stool sample is more than 30%.