# US 11,898,160 B2 · Method for maintaining pluripotency in cells

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- Number: US 11,898,160 B2
- Document ID: US11898160B2
- Status: Granted
- Kind: B2
- Application number: 12/577,103
- Filed: 2009-10-09
- Published: 2010-04-15
- Granted: 2024-02-13
- Earliest claimed: 2008-10-09
- Assignees: Minerva Biotechnologies Corporation
- Applicants: Cynthia C. Bamdad; Shawn P. Fessler
- Inventors: Cynthia C. Bamdad; Shawn P. Fessler
- Web page: https://patentgrep.com/patent/US11898160B2

## CPC

- C12N 5/0606: {Pluripotent embryonic cells, e.g. embryonic stem cells [ES] (embryonic germ cells C12N5/0611, induced pluripotent stem cells C12N5/0696)}
- C12N 5/0696: {Artificially induced pluripotent stem cells, e.g. iPS}
- C12N 2501/40: Regulators of development
- C12N 2501/50: Cell markers; Cell surface determinants
- C12N 2501/602: Sox-2
- C12N 2501/603: Oct-3/4
- C12N 2501/604: Klf-4
- C12N 2501/998: Proteins not provided for elsewhere
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The present application describes a method for inducing or maintaining pluripotency in a cell by contacting the cell with a biological or chemical species that increases MUC1* activity.

## Claims

### Claim 1

A method for maintaining cell pluripotency or cell stemness, the method comprising:
contacting a cell characterized by an expression of OCT4 with an effective amount of an exogenous nucleoside diphosphate protein kinase 7 (NME7),
thereby maintaining the expression of the OCT4 in the cell.

### Claim 2 (depends on 1)

The method of claim 1, wherein the NME7 is a polypeptide.

### Claim 3 (depends on 1)

The method of claim 1, wherein the cell is a stem cell.

### Claim 4 (depends on 1)

The method of claim 1, wherein the cell is an undifferentiated embryonic stem cell (ESC) or a pluripotent stem cell.

### Claim 5 (depends on 1)

The method of claim 1, wherein the cell is a human cell.

### Claim 6 (depends on 1)

The method of claim 1, wherein said contacting comprises contacting a plurality of cells that comprises the cell.

### Claim 7 (depends on 1)

The method of claim 1, wherein said contacting occurs in vitro.

### Claim 8 (depends on 1)

The method of claim 1, wherein said contacting occurs in the absence of one or more feeder cells or an extract thereof.

### Claim 9 (depends on 1)

The method of claim 1, wherein said contacting occurs in the absence of a basic fibroblast growth factor (bFGF).

### Claim 10 (depends on 1)

The method of claim 1, wherein the method stimulates or enhances the growth of the cell.

### Claim 11

A method for inducing pluripotency in a cell, the method comprising:
contacting the cell with transcription factors Oct4, Sox2, and Nanog, and
contacting the cell with an exogenous nucleoside diphosphate protein kinase 7 (NME7).

### Claim 12 (depends on 11)

The method of claim 11, wherein the NME7 is a polypeptide.

### Claim 13 (depends on 11)

The method of claim 11, wherein the cell is a fibroblast.

### Claim 14 (depends on 11)

The method of claim 11, wherein the cell is a human cell.

### Claim 15 (depends on 11)

The method of claim 11, wherein the method produces an induced pluripotent cell.

### Claim 16 (depends on 11)

The method of claim 11, wherein said contacting occurs in vitro.

### Claim 17 (depends on 11)

The method of claim 11, further comprising contacting the cell with one or more transcription factors selected from Klf4, c-Myc, and Lin28.

### Claim 18 (depends on 11)

The method of claim 11, wherein contacting the cell with transcription factors comprises introducing nucleic acids encoding said transcription factors into the cell.