# US 11,898,150 B2 · Brassica oleracea plants with improved nutritional value

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- Number: US 11,898,150 B2
- Document ID: US11898150B2
- Status: Granted
- Kind: B2
- Application number: 17/175,280
- Filed: 2021-02-12
- Published: 2021-09-02
- Granted: 2024-02-13
- Earliest claimed: 2012-09-13
- Assignees: Seminis Vegetable Seeds, Inc.
- Applicants: Seminis Vegetable Seeds, Inc.
- Inventors: Franciscus G. van den Bosch; Gerard N. Koorevaar
- Web page: https://patentgrep.com/patent/US11898150B2

## CPC

- C12N 15/8246: {Non-starch polysaccharides, e.g. cellulose, fructans, levans}
- A01H 1/02: Methods or apparatus for hybridisation; Artificial pollination {; Fertility}
- A01H 1/04: Processes of selection {involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection}
- A01H 5/02: Flowers
- A01H 5/10: Seeds
- A01H 6/203: {Brassica oleraceae, e.g. broccoli or kohlrabi}
- C12Q 1/6895: for plants, fungi or algae
- C12Q 2600/13: Plant traits
- C12Q 2600/156: Polymorphic or mutational markers
- C12Q 2600/158: Expression markers
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

The invention provides compositions and methods relating to the elevation of glucoraphanin compared to standard Brassica oleracea varieties. The invention also relates to the production of hybrid varieties having desired glucosinolate contents. The invention further provides plants, plant parts, and seeds comprising such traits and comprising a Myb28 allele from Brassica villosa that is not genetically linked to an ELONG allele from Brassica villosa.

## Claims

### Claim 1

A composition prepared from a Brassica oleracea plant with elevated glucosinolate content, or a part thereof, wherein the plant or part thereof comprises a chromosomal segment comprising a Myb28 allele from Brassica villosa and an ELONG allele from Brassica oleracea, wherein a sample of seed comprising said Myb28 allele and said ELONG allele was deposited under ATCC Accession No. PTA-13165, wherein said composition comprises at least one cell from said plant.

### Claim 2 (depends on 1)

The composition of claim 1, wherein the part thereof is defined as a floret, a seed, a cell, a root, a stem, a shoot, a leaf, a head, a flower, an anther, a petiole, pollen, a microspore, a haploid embryo, a somatic embryo, or a callus.

### Claim 3 (depends on 1)

The composition of claim 1, wherein the plant is a broccoli plant.

### Claim 4 (depends on 1)

The composition of claim 1, wherein a sample of seed comprising said chromosomal segment was deposited under ATCC Accession No. PTA-13165.

### Claim 5 (depends on 1)

The composition of claim 1, wherein the plant is inbred.

### Claim 6 (depends on 1)

The composition of claim 1, wherein the plant is hybrid.

### Claim 7 (depends on 1)

The composition of claim 1, wherein the plant is homozygous for said Myb28 allele from Brassica villosa.

### Claim 8 (depends on 1)

The composition of claim 1, wherein the plant is heterozygous for said Myb28 allele from Brassica villosa.