# US 11,898,140 B2 · Hyperthermophilic aerobic fermentation inoculant prepared by using municipal sewage sludge and its method

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- Number: US 11,898,140 B2
- Document ID: US11898140B2
- Status: Granted
- Kind: B2
- Application number: 16/826,261
- Filed: 2020-03-22
- Published: 2020-07-16
- Granted: 2024-02-13
- Earliest claimed: 2018-01-19
- Applicants: Fujian Agriculture and Forestry University
- Inventors: Shungui Zhou; Zhi Chen; Hanpeng Liao
- Web page: https://patentgrep.com/patent/US11898140B2

## CPC

- C12N 1/04: Preserving or maintaining viable microorganisms (immobilised microorganisms C12N11/00)
- C12N 1/20: Bacteria; Culture media therefor
- C05F 7/00: Fertilisers from waste water, sewage sludge, sea slime, ooze or similar masses
- C12N 1/205: Bacterial isolates
- C12N 2500/10: Metals; Metal chelators
- C12N 2500/34: Sugars
- C12R 2001/01: Bacteria or Actinomycetales {; using bacteria or Actinomycetales}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C05F: ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS

## Abstract

The present disclosure discloses a preparation method for hyperthermophilic aerobic fermentation inoculant prepared by using sewage sludge, the method includes the following steps: carrying out fermentation after the activation of hyperthermophilic aerobic bacteria, removing the supernatant from the fermentation products, and adding the protective agent and stirring until uniform, drying to obtain a product, pulverizing the product by a pulverizer, and sieving the product before sub-packing. The solution of the present disclosure has the following advantages.

## Claims

### Claim 1

A preparation method for a hyperthermophilic aerobic fermentation inoculant prepared by using sewage sludge, comprising:
fermenting hyperthermophilic aerobic bacteria to obtain a fermentation broth,
removing supernatant from the fermentation broth,
mixing the fermentation broth with protective agent to obtain a mixture,
drying the mixture,
pulverizing the dried mixture, and
sieving the pulverized mixture for packing;
wherein a culture medium for the fermentation is prepared by:
taking sewage sludge and adding water to the sewage sludge to regulate a solid content of the sewage sludge;
adding antifoaming agents of polyethers, yeast powder, ammonium chloride, sucrose, soluble starch, and potassium dihydrogen phosphate to the sewage sludge;
regulating pH of the sewage sludge;
adding composite carrier into the sewage sludge, and stirring the sewage sludge for uniformly mixing the composite carrier and the sewage sludge;
sterilizing the mixture of the composite carrier and the sewage sludge; and
cooling the sewage sludge to obtain the culture medium for the fermentation.

### Claim 2 (depends on 1)

The preparation method according to claim 1, wherein the protective agent is composed of non-fat milk powder, soluble starch, glycerin, and water.

### Claim 3 (depends on 1)

The preparation method according to claim 1, wherein the protective agent comprises 2 to 3 g non-fat milk powder, 1 to 2 g soluble starch, 10 mL glycerin, and 85 to 87 mL water.

### Claim 4 (depends on 1)

The preparation method according to claim 1, wherein the composite carrier is composed of at least one selected from the group consisting of kaolin, biochar, and wheat bran.

### Claim 5 (depends on 1)

The preparation method according to claim 1, wherein the content of the composite carrier is 50 to 100 g/L.

### Claim 6 (depends on 1)

The preparation method according to claim 1, wherein the solid content of the sludge is regulated to 2 to 3% by the addition of the water.

### Claim 7 (depends on 1)

The preparation method according to claim 1, wherein the composite carrier is composed of biochar and wheat bran, and a mass ratio of the biochar to the wheat bran is 1.5:1 to 3:1.

### Claim 8 (depends on 1)

The preparation method according to claim 1, wherein the hyperthermophilic aerobic bacteria is at least one selected from the group consisting of Thermus thermophilus, Calditerricola yamamurae, Calditerricola satsumensis, Thermaerobacter composti, Geobacillus thermocatenulatus or Thermaerobacter subterraneus.