# US 11,898,131 B2 · Cell culture substrate having a structural unit derived from furfuryl (meth) acrylate > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,898,131 B2 - Document ID: US11898131B2 - Status: Granted - Kind: B2 - Application number: 16/541,897 - Filed: 2019-08-15 - Published: 2020-02-20 - Granted: 2024-02-13 - Earliest claimed: 2018-08-16 - Assignees: Terumo Kabushiki Kaisha - Applicants: Terumo Kabushiki Kaisha - Inventors: Takao Anzai; Ichiro Hirahara - Web page: https://patentgrep.com/patent/US11898131B2 ## CPC - C12M 25/14: {Scaffolds; Matrices (in general C12N5/0068)} - C12M 23/20: {Material Coatings (immunocoatings C12M25/00)} - C12N 5/0068: {General culture methods using substrates (for specific animal cell type C12N5/06)} - C12N 2533/30: Synthetic polymers - C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS} - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) ## Abstract To provide a means capable of obtaining further excellent cellular adhesiveness than in a case where a surface of a cell culture substrate (cell culture vessel) is coated using polytetrahydrofurfuryl acrylate (PTHFA). Provided is a cell culture substrate comprising a coating layer on at least one side of a polymer substrate, wherein the coating layer includes a copolymer comprising more than 20% by mole and less than 100% by mole of a structural unit (1) derived from furfuryl (meth)acrylate represented by Formula (1) and more than 0% by mole and less than 80% by mole of a structural unit (2) derived from ethylenically unsaturated monomer having a hydroxyl group (the total of the structural unit (1) and the structural unit (2) is 100% by mole). ## Claims ### Claim 1 A cell culture substrate comprising a coating layer on at least one side of a polymer substrate, wherein the coating layer comprises a copolymer having a weight average molecular weight from 50,000 to 800,000 and consisting of at least 60% by mole and no more than 90% by mole of a structural unit (1) and at least 10% by mole and no more than 40% by mole of a structural unit (2), wherein the structural unit (1) is derived from a furfuryl acrylate monomer of Formula (1): wherein, R1 represents a hydrogen atom or a methyl group and R2 represents a group represented by the following Formula (1-1) or the following Formula (1-2): wherein, R3 represents an alkylene group having 1 to 3 carbon atoms, and wherein the structural unit (2) is derived from an ethylenically unsaturated monomer comprising an acryloyl or meth acryloyl group and a hydroxy group. ### Claim 2 (depends on 1) The cell culture substrate according to claim 1, wherein the ethylenically unsaturated monomer is a hydroxyalkyl acrylate monomer represented by Formula (2): wherein, R4 represents a hydrogen atom or a methyl group and R5 represents an alkylene group having 2 or 3 carbon atoms. ### Claim 3 (depends on 1) The cell culture substrate according to claim 1, wherein the polymer substrate is a porous membrane. ### Claim 4 (depends on 1) A bioreactor comprising the cell culture substrate according to claim 1. ### Claim 5 (depends on 4) A method for culturing a stem cell using the bioreactor according to claim 4. ### Claim 6 (depends on 2) The cell culture substrate according to claim 2, wherein the hydroxyalkyl acrylate monomer is selected from the group consisting of hydroxyethyl acrylate, hydroxypropyl acrylate, hydroxyisopropyl acrylate, hydroxyethyl methacrylate, hydroxypropyl methacrylate, hydroxyisopropyl methacrylate, and two or more combinations thereof. ### Claim 7 (depends on 6) The cell culture substrate according to claim 6, wherein the hydroxyalkyl acrylate monomer is hydroxyethyl methacrylate. ### Claim 8 (depends on 1) The cell culture substrate according to claim 1, wherein the copolymer has a weight average molecular weight from 150,000 to 350,000. ### Claim 9 (depends on 1) The cell culture substrate according to claim 1, wherein the coating layer has a thickness from 0.005 μm to 20 μm. ### Claim 10 (depends on 1) The cell culture substrate according to claim 1, wherein the coating layer consists of the copolymer. ### Claim 11 (depends on 1) The cell culture substrate according to claim 1, wherein the polymer substrate has one or more of a hollow fiber structure, a porous membrane structure, a sponge structure, and a flocculent structure. ### Claim 12 (depends on 1) The cell culture substrate according to claim 1, wherein the polymer substrate comprises hollow fibers having an inner diameter from 50 μm to 1000 μm and a length from 50 mm to 900 mm. ### Claim 13 (depends on 1) The cell culture substrate according to claim 1, wherein the polymer substrate comprises hollow fibers and wherein the coating layer is formed on one or both of an inner surface and an outer surface of the hollow fibers. ### Claim 14 (depends on 3) The cell culture substrate according to claim 3, wherein the porous membrane comprises hollow fibers. ### Claim 15 (depends on 1) The cell culture substrate according to claim 1, wherein the polymer substrate comprises a polymer selected from the group consisting of: polypropylene, polethylene, polystyrene, polysulfone, polyacrylonitrile, polytetrafluoroethylene, cellulose acetate, polyamide, polyarylethersulfone, polyvinylpyrrolidone, and mixtures of two or more thereof.