# US 11,897,947 B1 · ELISA for diagnosis of Haemonchus longistipes infection in camels

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 11,897,947 B1
- Document ID: US11897947B1
- Status: Granted
- Kind: B1
- Application number: 18/227,030
- Filed: 2023-07-27
- Granted: 2024-02-13
- Earliest claimed: 2022-09-09
- Assignees: King Faisal University
- Applicants: King Faisal University
- Inventors: Sabry Mohamed Bahy El-Bahr; El Awad M. El Hassan; Mohammad H. Al-Omran
- Web page: https://patentgrep.com/patent/US11897947B1

## CPC

- C07K 16/18: against material from animals or humans
- G01N 33/569: for microorganisms, e.g. protozoa, bacteria, viruses
- G01N 33/56966: {Animal cells}
- G01N 2333/4353: from nematodes
- G01N 2469/10: Detection of antigens from microorganism in sample from host
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

An ELISA for the diagnosis of Haemonchus longistipes infection in camels is provided. The ELISA for the diagnosis of Haemonchus longistipes infection in camels includes antibodies raised against a 76 kDa protein isolated from Haemonchus longistipes. The resulting antibodies may be used in assays to detect Haemonchus longistipes infected camels. The assays may be any kind of enzyme-linked immunosorbent assay, or “ELISA”, known to those of skill in the art. The assay using the antibodies raised against a 76 kDa protein isolated from Haemonchus longistipes infected camels may be capable of detecting pre-patent Haemonchus longistipes infection.

## Claims

### Claim 1

A method of treating camels for Haemonchus longistipes (H longistipes) infection, the method consisting of:
(1) obtaining a serum sample from a camel in need of screening for H. longistipes infection;
(2) exposing the serum sample to a purified H. longistipes specific antibody raised against an isolated and purified 76-kDa H. longistipes antigen;
(3) performing an assay to detect binding of the purified H. longistipes specific antibody to a 76-kDa H. longistipes antigen present in the serum sample; and
(4) if binding is detected between the H. longistipes specific antibody and the 76-kDa H. longistipes antigen present in the serum sample, administering a treatment for H. longistipes infection to the camel.

### Claim 2 (depends on 1)

The method of claim 1, wherein the H. longistipes infection is a pre-patent infection.

### Claim 3 (depends on 1)

The method of claim 1, wherein the assay is an Enzyme-Linked Immunosorbant Assay (ELISA).

### Claim 4 (depends on 3)

The method of claim 3, wherein the ELISA is selected from the group consisting of a Sandwich ELISA, a Competitive ELISA, and a Reverse ELISA.