# US 11,897,923 B2 · Panicum virgatum SOSEKI protein SOK2, coding gene and application thereof

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- Number: US 11,897,923 B2
- Document ID: US11897923B2
- Status: Granted
- Kind: B2
- Application number: 17/168,983
- Filed: 2021-02-05
- Published: 2022-05-19
- Granted: 2024-02-13
- Earliest claimed: 2021-02-05
- Assignees: Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences
- Applicants: Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences
- Inventors: Chunxiang Fu; Yamei Wang; Wenwen Liu; Zhenying Wu; Shiqie Bai; Chuan'en Zhou; Ruijuan Yang; Shanshan Jiang; Mengqi Wang
- Web page: https://patentgrep.com/patent/US11897923B2

## CPC

- C07K 14/415: from plants
- C12N 15/82: for plant cells {, e.g. plant artificial chromosomes (PACs)}
- C12N 15/8246: {Non-starch polysaccharides, e.g. cellulose, fructans, levans}
- C12N 15/8255: {involving lignin biosynthesis}
- C12N 15/8262: {involving plant development}
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The present invention relates to a coding gene of the SOSEKI protein SOK2 and an application thereof, wherein through molecular regulation of the SOSEKI protein SOK2, the flowering time of Panicum virgatum is delayed, biomass is increased, lignin content in the cell wall of Panicum virgatum is reduced and the fermentable sugar yield is boosted.

## Claims

### Claim 1

A cDNA encoding for a Panicum virgatum SOSEKI protein, said cDNA comprising SEQ ID No. 1.

### Claim 2 (depends on 1)

The cDNA of claim 1, wherein the SOSEKI protein comprises the amino acid sequence of SEQ ID No. 2.

### Claim 3 (depends on 1)

The cDNA of claim 1, wherein the nucleotide sequence is contained within the expression vector pANIC6B-PvSOK2.

### Claim 4

An expression vector to express the Panicum virgatum SOSEKI protein, wherein the expression vector is pANIC6B-PvSOK2, and wherein the PvSOK2 sequence is the nucleotide sequence SEQ ID NO. 1.