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US 11,897,918 B2Granted
Compositions and methods to increase production
AssigneeSeqirus UK Limited
InventorsSamuel Ho, David Parker, Peter Fekkes, Ivna De Souza, Peter Mason, Pirada Allen
Life of this patent
Estimating the term…
Abstract
Disclosed herein are methods for increasing protein yield and cellular productivity. Chemical agents facilitate host cell production of biological molecules to increase product yield.
Claims14
A method for producing influenza virus particles and influenza virus proteins from suspension MDCK cells comprising:
- growing or culturing the suspension MDCK cells for a duration of time, at least a portion of which is carried out in the presence of at least one chemical agent selected from the group consisting of fluvastatin, atorvastatin, cerivastatin, lovastatin, mevastatin, pravastatin or isomers thereof;
- harvesting the influenza virus particles and influenza virus proteins from the suspension MDCK cells;
- and
- formulating the influenza virus particles and influenza virus proteins into a pharmaceutical composition;
- wherein the at least one chemical agent has a concentration of 0.001 μM to 5 μM in culture.
2 more elements
A method for producing influenza virus particles or influenza virus proteins from suspension MDCK cells comprising:
- growing or culturing the suspension MDCK cells for a duration of time, at least a portion of which is carried out in the presence of at least one chemical agent selected from the group consisting of fluvastatin, atorvastatin, cerivastatin, lovastatin, mevastatin, pravastatin or isomers thereof;
- harvesting the influenza virus particles or influenza virus proteins from the suspension MDCK cells;
- and
- formulating the influenza virus particles or influenza virus proteins into a pharmaceutical composition;
- wherein the at least one chemical agent has a concentration of 0.001 μM to 5 μM in culture.
2 more elements
Dependencies are inferred from the claim text.