# US 11,896,975 B2 · System for implementing biological or chemical methods > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,896,975 B2 - Document ID: US11896975B2 - Status: Granted - Kind: B2 - Application number: 16/965,442 - Filed: 2019-01-30 - Published: 2021-02-25 - Granted: 2024-02-13 - Earliest claimed: 2018-02-02 - Assignees: Scienova GmbH - Applicants: Scienova GmbH - Inventors: Stefan Kreusch; Thomas Richter; Raffael Rubick - Web page: https://patentgrep.com/patent/US11896975B2 ## CPC - B01L 3/50825: {Closing or opening means, corks, bungs} - B01L 3/50853: {with covers or lids (closures for test tubes B01L3/50825)} - B01L 2200/0689: Sealing - B01L 2300/023: Sending and receiving of information, e.g. using Bluetooth® - B01L 2300/046: Function or devices integrated in the closure - B01L 2300/048: enabling gas exchange, e.g. vents - B01L 2300/0618: for removable separation walls - B01L 2300/0663: Whole sensors - B01L 2300/0681: Filter - B01L 2300/0858: Side walls - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE ## Abstract The invention having functional vertical disposable reaction systems having a vertically mounted semipermeable membrane for sample preparation, chemical reactions, dialysis, enzymatic/microbiological fermentation, multistage processes, in vitro protein biosynthesis on a laboratory scale, formed from a base body and an exchangeable lid having different functions. For exchange across the membrane, the system is placed vertically into an outer volume consisting of gas, liquid or solid constituents. The system consists of a dimensionally stable base body and a liquid-tight lid having a functional support going toward the base of the base body, the dimensionally stable base body forming at least one noncapillary reaction space as inner volume with at least one semipermeable membrane as lateral wall. The high flexibility in use results from the combination of variants of the base bodies with different lid variants for different areas of use. The base bodies having different membranes and volumes can be coupled with lids having different feeding openings, contacts, sensor supports, gas supply means, circulation means, etc. This yields, in the case of m different base bodies and n different lid variants, m×n combinations having different properties. ## Claims ### Claim 1 A system for carrying out biological or chemical methods, comprising: at least one base body, and at least one separately provided lid which is matched with the base body such that lid and base body can form a firm connection with one another, wherein the base body comprises at least one structural element and at least one semipermeable membrane and the semipermeable membrane at least sectionally borders an inner volume of the base body as a lateral wall running substantially in parallel to the longitudinal axis of the base body, wherein the lid comprises at least one functional support which is, at its proximal end, connected to a sealing section of the lid and comprises, in the region of its distal end, one or more functional features, wherein the inner volume has a proximal section which is, upon connection of lid and base body, arranged in the proximity of the sealing section and a distal section which has, upon connection of lid and base body, a distance from the sealing section that is at least 90% of the maximal distance from the sealing section within the inner volume, wherein the distal end of the functional support is, upon connection of lid and base body, arranged in the distal section, wherein the functional features are suitable for capturing, changing and/or influencing states of biological or chemical methods. ### Claim 2 (depends on 1) The system as claimed in claim 1, wherein the base body has an opening of the inner volume that is, upon connection of the base body to the lid, closed by the sealing section thereof. ### Claim 3 (depends on 1) The system as claimed in claim 1, wherein the inner volume is from 50 μl to 200 ml. ### Claim 4 (depends on 1) The system as claimed in claim 1, wherein a functional feature is a sensor. ### Claim 5 (depends on 1) The system as claimed in claim 1, wherein a functional feature is a gas-supply opening, filling opening r removal opening, or a combination thereof. ### Claim 6 (depends on 1) The system as claimed in claim 1, wherein a gas-tight closure is achieved by connection of lid and base body. ### Claim 7 (depends on 1) The system as claimed in claim 1, wherein the lid comprises at least one positioning element for connection of the lid to further lids, to an outer vessel or to a float. ### Claim 8 (depends on 1) The system as claimed in claim 1, wherein the lid comprises multiple functional supports having different functional features. ### Claim 9 (depends on 1) The system as claimed in claim 1, wherein the lid comprises contacting elements, especially for supply of power and/or transmission of data. ### Claim 10 (depends on 1) The system as claimed in claim 1, wherein the inner volume is from 1000 μl to 50 ml. ### Claim 11 (depends on 4) The system as claimed in claim 4, wherein the functional feature is a temperature sensor or a conductivity sensor or a combination thereof. ### Claim 12 (depends on 6) The system as claimed in claim 6, wherein the gas-tight closure is achieved by a lip seal, an elastic seal or adhesive bonding, potting, welding or overmolding. ### Claim 13 (depends on 1) The system as claimed in claim 1, wherein the semipermeable membrane at least sectionally borders the inner volume of the base body as a lateral wall thereof running substantially in parallel to the longitudinal axis of the base body. ### Claim 14 (depends on 1) The system as claimed in claim 1, wherein the semipermeable membrane at least sectionally borders the inner volume of the base body as a lateral wall running substantially in parallel to the longitudinal axis of the base body in order to allow material exchange with an outer volume across the semipermeable membrane. ### Claim 15 (depends on 1) A method for carrying out biological or chemical methods using the system as claimed in claim 1, comprising the steps: providing an outer volume, providing a base body, selecting a suitable lid with respect to the functional features thereof, connecting base body and lid, inserting base body with lid into the outer volume.