# US 11,896,973 B2 · Thermal activated microfluidic switching

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- Number: US 11,896,973 B2
- Document ID: US11896973B2
- Status: Granted
- Kind: B2
- Application number: 17/211,480
- Filed: 2021-03-24
- Published: 2021-07-08
- Granted: 2024-02-13
- Earliest claimed: 2014-05-16
- Assignees: Cytonome/St, LLC
- Applicants: Cytonome/St, LLC
- Inventors: Donald Francis Perrault, Jr.; Johnathan Charles Sharpe; Erin Koksal
- Web page: https://patentgrep.com/patent/US11896973B2

## CPC

- B01L 3/502738: {characterised by integrated valves (throttle valves in microfluidic sample containers B01L3/502746)}
- B01L 3/502761: {specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules}
- G01N 21/00: Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light (G01N3/00 - G01N19/00 take precedence)
- G01N 35/00: Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- B01L 3/5027: {by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip}
- B01L 2200/0652: Sorting or classification of particles or molecules
- B01L 2300/0816: Cards, e.g. flat sample carriers usually with flow in two horizontal directions
- B01L 2300/0864: comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
- B01L 2400/0442: thermal energy, e.g. vaporisation, bubble jet
- B01L 2400/0487: fluid pressure, pneumatics
- B01L 2400/0622: distribution valves, valves having multiple inlets and/or outlets, e.g. metering valves, multi-way valves
- G01N 2015/1028: {Sorting particles}
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

A microfluidic chip assembly having a plurality of microfluidic flow channels is provided. Each channel has a switching region. The microfluidic chip may further include at least one bubble jet actuator configured to generate a pressure pulse in the switching regions of the channels to selectively deflect particles in the flow. The bubble jet actuator may be configured as a blind chamber, as an operative non-through flow chamber and/or as a self-replenishment chamber. The bubble jet actuator may include a trapped air bubble. The bubble jet actuator may include a plurality of heating elements individually controlled for pre-nucleation warmup and/or for triggering vapor bubble nucleation.

## Claims

### Claim 1

A microfluidic particle processing chip assembly comprising;
a substrate;
a flow channel formed in the substrate, the flow channel having:
a focusing region for focusing a flow of particles within the flow channel;
an interrogation region at least partially downstream of the focusing region;
a switching region at least partially downstream of the interrogation region;
a first output channel; and
a second output channel;
a first side channel formed in the substrate and fluidically coupled to the flow channel;
a second side channel formed in the substrate and fluidically coupled to the flow channel opposite the first side channel;
a first bubble jet actuator fluidically coupled to the first side channel, the first bubble jet actuator including one or more heating elements disposed in the first side channel and configured to generate a pressure pulse in the flow in the switching region of the flow channel to switch particles into one of the first output channel or the second output channel; and
a second bubble jet actuator fluidically coupled to the second side channel, the second bubble jet actuator including one or more heating elements disposed in the second side channel and configured to generate a pressure pulse in the flow of the switching region of the flow channel to switch particles into one of the first output channel or the second output channel.

### Claim 2 (depends on 1)

The microfluidic chip assembly of claim 1, further comprising an air purge line in fluid communication with at least one of the first bubble jet actuator and the second bubble jet actuator, the first bubble jet actuator or the second bubble jet actuator is configured to be vented through the air purge line during an initialization operation.

### Claim 3 (depends on 1)

The microfluidic chip assembly of claim 1, wherein the first bubble jet actuator comprises an operative non-through flow chamber or a self-replenishment chamber.

### Claim 4 (depends on 1)

The microfluidic chip assembly of claim 1, wherein the flow channel further comprises a third output channel such that particles flow into the third output channel when no pressure pulse is applied by the first bubble jet actuator or the second bubble jet actuator.

### Claim 5 (depends on 1)

The microfluidic chip assembly of claim 1, wherein each of the one or more heating elements of the first bubble jet actuator is controllable by a signal generator.

### Claim 6 (depends on 5)

The microfluidic chip assembly of claim 5, wherein each of the one or more heating elements of the first bubble jet actuator is controllable for pre-nucleation warmup and/or for triggering vapor bubble nucleation.

### Claim 7 (depends on 5)

The microfluidic chip assembly of claim 5, wherein each of the one or more heating elements of the first bubble jet actuator is controllable for triggering vapor bubble nucleation.

### Claim 8 (depends on 1)

The microfluidic chip assembly of claim 1, wherein the first bubble jet actuator includes a trapped air bubble.

### Claim 9 (depends on 8)

The microfluidic chip assembly of claim 8, wherein the first bubble jet actuator includes a constricted region between at least one heating element and the trapped air bubble.

### Claim 10 (depends on 1)

The microfluidic chip assembly of claim 1, wherein the second bubble jet actuator acts as a pressure pulse dampening element with respect to the first bubble jet actuator.

### Claim 11 (depends on 1)

The microfluidic chip assembly of claim 1, wherein the flow channel is one of a plurality of flow channels formed in the substrate, each of the plurality of flow channels including a pair of the first and second bubble jet actuators formed on or in the substrate, the pair of the first and the second bubble jet actuators is configured to generate opposing pressure pulses in the switching region of a respective one of the plurality of flow channels to switch particles into the respective first output channel or second output channel.

### Claim 12 (depends on 11)

The microfluidic chip assembly of claim 11, wherein the plurality of flow channels are merged or aggregated in the substrate.