# US 11,896,738 B2 · Biomimetic electrically conductive hyaluronic acid-based hydrogels

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 11,896,738 B2
- Document ID: US11896738B2
- Status: Granted
- Kind: B2
- Application number: 17/198,211
- Filed: 2021-03-10
- Published: 2021-09-16
- Granted: 2024-02-13
- Earliest claimed: 2020-03-12
- Assignees: The Regents of the University of California
- Applicants: The Regents of the University of California
- Inventors: Rebecca Bierman
- Web page: https://patentgrep.com/patent/US11896738B2

## CPC

- A61L 27/52: Hydrogels or hydrocolloids
- A61N 1/0529: {Electrodes for brain stimulation}
- A61L 27/08: Carbon {; Graphite}
- A61L 27/20: Polysaccharides
- A61L 27/34: Macromolecular materials
- A61L 27/3834: {Cells able to produce different cell types, e.g. hematopoietic stem cells, mesenchymal stem cells, marrow stromal cells, embryonic stem cells}
- A61L 27/3878: {Nerve tissue, brain, spinal cord, nerves, dura mater}
- A61L 27/443: {with carbon fillers}
- A61L 27/50: Materials characterised by their function or physical properties {, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials}
- A61N 1/0551: {Spinal or peripheral nerve electrodes}
- A61N 1/0553: {Paddle shaped electrodes, e.g. for laminotomy}
- C08J 3/075: Macromolecular gels
- C08K 3/042: {Graphene or derivatives, e.g. graphene oxides}
- C08K 3/046: {Carbon nanorods, nanowires, nanoplatelets or nanofibres}
- A61L 2400/12: Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces
- A61L 2400/18: Modification of implant surfaces in order to improve biocompatibility, cell growth, fixation of biomolecules, e.g. plasma treatment
- C08J 2305/08: Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof
- C08K 7/06: Elements
- C08K 7/24: inorganic
- C08K 2201/001: Conductive additives
- A61L: METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agents employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toiletry purposes A61K)
- A61N: ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY (measurement of bioelectric currents A61B; surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body A61B18/00; anaesthetic apparatus in general A61M; incandescent lamps H01K; infrared radiators for heating H05B)
- C08J: WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H (working, e.g. shaping, of plastics B29)
- C08K: Use of inorganic or non-macromolecular organic substances as compounding ingredients (paints, inks, varnishes, dyes, polishes, adhesives C09)

## Abstract

An electrically conductive hyaluronic acid-based hydrogel is disclosed that is a crosslinked porous scaffold having a graphene-based material encapsulated or in contact within the porous scaffold. The graphene-based material includes one or more of graphene oxide foam, reduced graphene oxide foam, nanoplatelets, nanoparticles, or fibers. The porous scaffold may be formed over an implanted bioelectronic device such as a microelectrode array having a plurality of electrodes. The porous scaffold may also be used to control the differentiation of cells including Neural Stem/Progenitor Cells (NS/PCs).

## Claims

### Claim 1

An implantable bioelectronic device comprising a plurality of electrodes, the device comprising a polymer coated body and a plurality of metallic electrodes, wherein an electrically conductive hyaluronic acid-based hydrogel crosslinked into a porous scaffold is adhered to the polymer coated body and wherein the crosslinked porous scaffold is punctuated with holes or apertures located adjacent to the plurality of metallic electrodes, wherein the holes or apertures contain a graphene-based material therein.

### Claim 2 (depends on 1)

The implantable bioelectronic device of claim 1, wherein the porous scaffold is formed by thiolated hyaluronic acid chains (HA-SH), multi-arm polyethylene glycol (PEG) thiol (PEG-SH), and multi-arm polyethylene glycol maleimide (PEG-Mal).

### Claim 3 (depends on 1)

The implantable bioelectronic device of claim 1, wherein the porous scaffold is formed by methacrylated hyaluronic acid chains (MeHA), multi-arm polyethylene glycol (PEG) thiol (PEG-SH), and multi-arm polyethylene glycol norbornene (PEG-Norb).

### Claim 4 (depends on 1)

The implantable bioelectronic device of claim 1, further comprising one or more of: a peptide, a protein or fragment thereof, a cell adhesion peptide, or a therapeutic drug disposed on or within the crosslinked porous scaffold.

### Claim 5 (depends on 1)

The implantable bioelectronic device of claim 1, wherein the graphene-based material comprises graphene oxide foam or reduced graphene oxide foam.

### Claim 6 (depends on 1)

The implantable bioelectronic device of claim 1, wherein the graphene comprises a graphene oxide poly(ε-caprolactone) (GO-PCL) scaffold or fibers in contact with the crosslinked porous scaffold.

### Claim 7 (depends on 1)

The implantable bioelectronic device of claim 1, wherein the graphene-based material is reduced.