# US 11,896,707 B2 · Cosmetic preparations comprising Daphne extracts > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,896,707 B2 - Document ID: US11896707B2 - Status: Granted - Kind: B2 - Application number: 17/312,538 - Filed: 2019-12-10 - Published: 2022-02-24 - Granted: 2024-02-13 - Earliest claimed: 2018-12-10 - Assignees: ETH Zurich; UniversitäT Basel - Applicants: ETH Zurich; Universität Basel - Inventors: Michael Detmar; Epameinondas Gousopoulos; Olivier Potterat; Jihye Kim; Adriana Sliwa-Primorac; Antonia Elisabeth Schantl; Maria Teresa Faleschini; Matthias Hamburger - Web page: https://patentgrep.com/patent/US11896707B2 ## CPC - A61K 8/63: Steroids; Derivatives thereof - A61K 8/9789: Magnoliopsida [dicotyledons] - A61K 8/4973: {with oxygen as the only hetero atom} - A61Q 19/08: Anti-ageing preparations - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - A61Q: SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS ## Abstract The present invention relates to the use of plant extracts from the genus Daphne for the cosmetic treatment of the skin and to cosmetic preparations which comprise extracts of Daphne giraldii and/or Daphne gnidium or other members of the genus Daphne containing daphnetoxin and/or other daphnane diterpenoids for skin treatment. The plant extracts and cosmetic preparations are particularly useful for stimulation of the lymphatic system. ## Claims ### Claim 1 A cosmetic preparation for skin treatment, wherein the preparation comprises: plant extracts from Daphne giraldii and/or from Daphne gnidium; and said plant extracts contain daphnane diterpenoids in an amount of 0.00001-1 wt % based on the total weight of the cosmetic preparation. ### Claim 2 (depends on 1) The preparation of claim 1, wherein said plant extracts are present in an amount of 0.0001-10 wt % in case of crude plant extracts, or 0.00001-5 wt % in case of enriched extracts, or 10 pM-10 mM in case of purified substances of said plant extracts based on the total weight of the preparation; are obtained by an extraction method using at least one of a solvent and a fluid selected from the group consisting of: a polar solvent, an apolar solvent, and a supercritical fluid, and/or mixtures of CO2 with C1-C3 alcohols or with acetone; and/or are obtained from one or more of: fresh extracts of the plant's roots, fresh extracts of the plant's green part, dried extracts of the plant's roots, or dried extracts of the plant's green part. ### Claim 3 (depends on 1) The preparation of claim 1, further comprising one or more cosmetic excipients selected from the group consisting of emollients, moisturizers, thickeners, emulsifiers, colorings, detergents, disinfectants, antioxidants, buffers, matting agents, exfoliating agents, aromas, essential oils, vitamins, UV filters, and extracellular matrix components. ### Claim 4 (depends on 1) The preparation according to claim 1 formulated as a liquid formulation; or a semisolid or solid formulation. ### Claim 5 (depends on 1) The preparation of claim 1, wherein the daphnane diterpenoids are daphnetoxin. ### Claim 6 (depends on 2) The preparation of claim 2, wherein the polar solvent is selected from the group consisting of C1-C4 alcohols, C1-C4 diols, glycerol, acetone, and ethyl acetate, in each case optionally in the presence of water. ### Claim 7 (depends on 2) The preparation of claim 2, wherein the apolar solvent is selected from the group consisting of C4-C10 alkanes, C1-C2 alkylhalogenides, and C1-C4 dialkylethers. ### Claim 8 (depends on 2) The preparation of claim 2, wherein the supercritical fluid is CO2. ### Claim 9 (depends on 2) The preparation of claim 2, wherein the polar solvent is used in the extraction method. ### Claim 10 (depends on 3) The preparation of claim 3, wherein the extracellular matrix components are selected from the group consisting of collagen, elastin, and hyaluronic acid. ### Claim 11 (depends on 4) The preparation of claim 4, wherein the liquid formulation is a gel, a lotion, a milk, an emulsion, a foam, a spray, or an oil. ### Claim 12 (depends on 4) The preparation of claim 4, wherein the semisolid or solid formulation is a cream, an ointment, a stick, or a powder. ### Claim 13 (depends on 1) A method of cosmetically treating the skin, wherein the method comprises applying to skin in need thereof a preparation according to claim 1. ### Claim 14 (depends on 13) The method of claim 13, wherein skin is treated to stimulate the lymphatic system. ### Claim 15 (depends on 13) The method of claim 13, wherein the skin is treated for rejuvenation of the skin, anti-aging of the skin, puffy-eye treatment, detoxification of the skin, increased firmness of the skin, and/or anti-swelling effect. ### Claim 16 (depends on 13) The method of claim 13, where said treatment increases the longevity of skin cells; counteracts intrinsic and/or extrinsic skin ageing; protects the skin; and/or counteracts swelling of the skin. ### Claim 17 A method of stimulating the lymphatic system, the method comprising applying an effective amount of a preparation to the skin of a subject in need thereof, wherein said preparation comprises: plant extracts from Daphne giraldii and/or from Daphne gnidium; and said plant extracts contain daphnane diterpenoids, in an amount of 0.00001-1 wt % based on the total weight of the preparation. ### Claim 18 (depends on 17) The method of claim 17, wherein the daphnane diterpenoids are daphnetoxin.