# US 11,896,685 B2 · Core-shell network structure comprising biopolymer and composition comprising same > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,896,685 B2 - Document ID: US11896685B2 - Status: Granted - Kind: B2 - Application number: 17/290,131 - Filed: 2019-10-31 - Published: 2021-12-23 - Granted: 2024-02-13 - Earliest claimed: 2018-10-31 - Assignees: Amorepacific Corporation - Applicants: Amorepacific Corporation - Inventors: Eunmi Kim; Young Gyu Kang; Jaeyoung Ko; Miju Kim; Minkee Kim; Yong Jin Kim; Heungsoo Baek; Jaewon You; Yonghee Lee; Eun Soo Lee; Yuri Choi - Web page: https://patentgrep.com/patent/US11896685B2 ## CPC - A61K 8/0233: {Distinct layers, e.g. core/shell sticks} - A61Q 19/08: Anti-ageing preparations - A61K 8/11: Encapsulated compositions - A61K 8/64: Proteins; Peptides; Derivatives or degradation products thereof - A61K 8/645: {Proteins of vegetable origin; Derivatives or degradation products thereof} - A61K 8/73: Polysaccharides - A61Q 19/00: Preparations for care of the skin - A61K 2800/412: Microsized, i.e. having sizes between 0.1 and 100 microns - A61K 2800/594: Mixtures of polymers - A61K 2800/622: by organic compounds - A61K 2800/652: The particulate/core comprising organic material - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - A61Q: SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS ## Abstract The present invention relates to a core-shell network structure formed using a biocompatible biopolymer and to a composition comprising same. Said structure can be used as an alternative to synthetic chemical materials, such as polyethylene glycol (PEG), to effectively disperse an insoluble effective substance within a composition, thus addressing safety issues, and formulations can be preserved stably for a long time through said network structure. Furthermore, said structure and the composition comprising same may provide useful benefits for the skin in terms of skin barrier enhancement, skin moisturization, skin regeneration, or the like. ## Claims ### Claim 1 A core-shell network structure comprising a core-shell particle formed of: a core comprising prolamin; and a shell comprising pullulan and pectin, wherein an interconnected network is formed between the core-shell particles as the pullulan surrounds the core and the pectin is located at an outermost layer of the shell. ### Claim 2 (depends on 1) The core-shell network structure according to claim 1, wherein the prolamin comprises one or more selected from a group consisting of zein, hordein, secalin, kafirin, gliadin, oryzin and avenin. ### Claim 3 (depends on 1) The core-shell network structure according to claim 1, wherein the structure comprises: 0.025-7.5 wt % of prolamin; 0.025-12.5 wt % of pullulan; and 0.05-10 wt % of pectin, based on the total weight of the structure. ### Claim 4 (depends on 1) The core-shell network structure according to claim 1, wherein the core-shell particle has an average particle size of greater than 100 nm and 600 nm or smaller. ### Claim 5 (depends on 1) A method for one or more of skin barrier enhancement, skin moisturization and skin regeneration, which comprises administering an effective amount of the structure according to claim 1 to a subject in need thereof. ### Claim 6 (depends on 5) The method according to claim 5, wherein the structure is comprised in a composition as an active ingredient, wherein the composition comprises the structure in an amount of 0.005 wt % or more and less than 1 wt % based on the total weight of the composition. ### Claim 7 (depends on 5) The method according to claim 5, wherein an administration dosage of structure is 1-500 mg/kg/day. ### Claim 8 (depends on 1) A composition comprising the structure according to claim 1, which further comprises an insoluble effective substance in the core of the structure, wherein the insoluble effective substance is captured by the prolamin of the core. ### Claim 9 (depends on 8) The composition according to claim 8, wherein the insoluble effective substance is a hydrophobic and alcohol-soluble substance. ### Claim 10 (depends on 8) The composition according to claim 8, wherein the prolamin of the core forms a brick-like structure around the insoluble effective substance. ### Claim 11 (depends on 8) The composition according to claim 8, wherein the insoluble effective substance comprises: a triterpenoid comprising one or more selected from a group consisting of oleanolic acid, ursolic acid and arjunolic acid; a polyphenol or a polyphenol derivative comprising one or more selected from a group consisting of amentoflavone, ellagic acid, apigenin, bergenin, diosmetin, univestin, resveratrol, isoflavone and catechin; an oily fatty acid comprising one or more selected from a group consisting of salicylic acid, α-lipoic acid, caffeine, tocopherol, docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA) and conjugated linolenic acid (CLA); a sphingolipid comprising one or more selected from a group consisting of sphingomyelin, ganglioside, cerebroside, ceramide, glycosyl ceramide, lactosyl ceramide, galactosyl ceramide and xylosyl ceramide; a saponin comprising compound K (20-O-β-D-glucopyranosyl-20(S)-protopanaxadiol); carotene or a carotene derivative; and a natural extract comprising one or more selected from a group consisting of ginkgo leaf extract and red ginseng extract. ### Claim 12 (depends on 8) The composition according to claim 8, wherein the composition comprises: 0.01-3 wt % of prolamin; 0.01-5 wt % of pullulan; and 0.01-2 wt % of pectin, based on the total weight of the composition. ### Claim 13 (depends on 8) The composition according to claim 8, wherein the insoluble effective substance is comprised in an amount of 0.01-10 wt % based on the total weight of the composition. ### Claim 14 (depends on 8) A method for one or more of skin barrier enhancement, skin moisturization and skin regeneration, which comprises administering an effective amount of the composition according to claim 8 to a subject in need thereof. ### Claim 15 (depends on 8) The method according to claim 8, wherein the composition is a cosmetic composition. ### Claim 16 (depends on 1) A method for preparing the structure according to claim 1, comprising: a step of forming a core by dispersing prolamin in an alcohol solvent; a step of forming a shell surrounding the core by adding pullulan onto the alcohol solution in a drop-by-drop manner; a step of coating pectin on an outermost layer of the shell and forming a network between the shell by adding pectin to the pullulan-added alcohol solution; and a step of obtaining a solution with a core-shell network structure dissolved in an aqueous phase formed by evaporating the alcohol from the pectin-added solution. ### Claim 17 (depends on 1) A method for stabilizing an insoluble effective substance by the structure according to claim 1, comprising: a step of forming a core by dispersing prolamin and an insoluble effective substance in an alcohol solvent; a step of forming a shell surrounding the core by adding pullulan onto the alcohol solution in a drop-by-drop manner; a step of coating pectin on an outermost layer of the shell and forming a network between the shells by adding pectin to the pullulan-added alcohol solution; and a step of obtaining a solution with a core-shell network structure dissolved in an aqueous phase by evaporating the alcohol from the pectin-added solution. ### Claim 18 (depends on 17) The method according to claim 17, wherein the step of forming the network between the shells further comprises a step of gelating the solution with the core-shell network structure formed by adjusting the acidity of the alcohol solution to pH 2.5-6.5. ### Claim 19 (depends on 17) The method according to claim 17, wherein the temperature in the step of evaporating the alcohol is 20-40° C. ### Claim 20 (depends on 17) The method according to claim 17, wherein the insoluble effective substance is a hydrophobic and alcohol-soluble substance.