# US 11,896,680 B2 · Detection method > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,896,680 B2 - Document ID: US11896680B2 - Status: Granted - Kind: B2 - Application number: 16/762,906 - Filed: 2018-11-27 - Published: 2021-06-10 - Granted: 2024-02-13 - Earliest claimed: 2017-11-28 - Assignees: Mie University - Applicants: Mie University - Inventors: Akira Mizoguchi; Koji Tanaka; Kazushi Kimura; Tetsuya Nosaka; Kyosuke Tanaka; Shujie Wang; Aika Kaito; Kousyoku Sai; Yuji Toiyama; Hidemasa Goto; Masahiko Sugimoto; Yuuhei Nishimura - Web page: https://patentgrep.com/patent/US11896680B2 ## CPC - A61K 49/006: {Biological staining of tissues in vivo, e.g. methylene blue or toluidine blue O administered in the buccal area to detect epithelial cancer cells, dyes used for delineating tissues during surgery} - A61B 1/00006: {of control signals} - A61B 1/000094: {extracting biological structures} - A61B 1/00055: {for alerting the user} - A61B 1/00172: {with means for scanning} - A61B 1/043: {for fluorescence imaging} - A61B 1/045: Control thereof - A61B 1/063: {for monochromatic or narrow-band illumination} - A61B 1/31: for the rectum, e.g. proctoscopes or sigmoidoscopes - A61B 5/0071: {by measuring fluorescence emission} - G01N 21/6486: {Measuring fluorescence of biological material, e.g. DNA, RNA, cells (G01N21/6428 takes precedence)} - G01N 21/6458: {Fluorescence microscopy (fluorescence microscopes per se G02B21/0076 and G02B21/16)} - G01N 2021/6439: {with indicators, stains, dyes, tags, labels, marks} - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - A61B: DIAGNOSIS; SURGERY; IDENTIFICATION (usefulness limited to only animals A61D) - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract Provided is a vital stain inspection method for distinguishing between cancer cells and normal cells within a lumen, or on the serous membrane side of an organ, by means of laser irradiation after organ tissue has been stained by curcumin, Red #3 or Red #106, which are edible color dyes. ## Claims ### Claim 1 A method for detecting cancer progression comprising staining an organ tissue suspected of having cancer with curcumin and then laser irradiating the organ tissue from serosal side or lumen using a multiphoton laser microscopic endoscope, confocal laser microscopic endoscope or laser fluorescent microscopic endoscope, and visualizing Meissner's plexus or Auerbach's plexus present in the organ tissue, wherein the organ tissue is digestive tract tissue, and a primary lesion is in mucosal epithelium. ### Claim 2 (depends on 1) The method according to claim 1, wherein if cancer cells have invaded or reached Meissner's plexus, the cancer is an advanced cancer. ### Claim 3 (depends on 1) The method according to claim 1, wherein if cancer cells have invaded or reached the Meissner's plexus and smooth muscle layer, the cancer is an advanced cancer. ### Claim 4 (depends on 1) The method according to claim 1, wherein if cancer cells have not invaded or reached Meissner's plexus, the cancer is an early cancer. ### Claim 5 (depends on 1) The method according to claim 1, which further comprises signaling the detection of cancer cells by sound or light. ### Claim 6 (depends on 1) A method of claim 1 for treating cancer patients comprising removing cancer cells one by one from serosal side or lumen. ### Claim 7 (depends on 1) A method of claim 1 for removing the cancer cells one-by-one, comprising confirming cancer cells remaining in a living body from serosal side or lumen after surgery. ### Claim 8 (depends on 1) A method according to claim 1 for treating colon cancer patients. ### Claim 9 (depends on 1) A method according to claim 1 for treating lung cancer patients. ### Claim 10 (depends on 1) A method according to claim 1 for treating prostate cancer patients. ### Claim 11 (depends on 1) A method according to claim 1 for treating gastric cancer patients. ### Claim 12 (depends on 1) A method according to claim 1 for treating esophageal cancer patients. ### Claim 13 (depends on 1) A method according to claim 1 for treating bladder cancer patients. ### Claim 14 (depends on 1) A method according to claim 1 for diagnosing advanced cancer, wherein the cancer is judged as an advanced cancer based on visualized images if the cancer cells have invaded or reached Meissner's plexus, or the cancer cells have invaded or reached Meissner's plexus and smooth muscle layer. ### Claim 15 (depends on 1) A method according to claim 1 for diagnosing early cancer, wherein the cancer is judged as an early cancer based on visualized images if the cancer cells have not invaded or reached Meissner's plexus. ### Claim 16 (depends on 1) A method according to claim 1 for treating patients with uterine cancer or ovarian cancer. ### Claim 17 (depends on 1) A method according to claim 1 for treating patients with breast cancer. ### Claim 18 (depends on 1) A method according to claim 1 for treating patients with pancreatic cancer or gallbladder cancer. ### Claim 19 (depends on 1) A method according to claim 1 for treating tongue cancer, throat cancer, laryngeal cancer, or thyroid cancer.