RSV vaccines and methods of production and use thereof
AssigneeThe Board of Regents for Oklahoma State University
InventorAntonius G. P. Oomens
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Abstract
Recombinant, live, attenuated viruses of the Pneumoviridae family are disclosed that include a baculovirus GP64 envelope glycoprotein or variant or fragment thereof and a respiratory syncytial virus (RSV) F protein variant or fragment thereof. Also disclosed are polynucleotides encoding the virus as well as pharmaceutical compositions and vaccines containing the virus. In addition, methods of producing and using each of the above compositions are also disclosed.
Claims22
An isolated polynucleotide encoding at least a portion of an infection-attenuated virus of the Pneumoviridae family, the polynucleotide comprising:
- a gene encoding a baculovirus GP64 envelope glycoprotein or variant or fragment thereof;
- and
- a gene encoding an RSV F protein variant or fragment thereof that comprises at least one amino acid substitution compared to a native RSV F protein, wherein the at least one amino acid substitution stabilizes the RSV F protein variant or fragment thereof in a pre-fusion conformation;
- and
- wherein the genome comprises at least one additional modification that renders the virus infection-attenuated.
A mammalian cell, comprising:
- at least one polynucleotide encoding a baculovirus GP64 envelope glycoprotein or variant or fragment thereof;
- and
- at least one polynucleotide encoding at least a portion of a genome of an infection-attenuated virus of the Pneumoviridae family, wherein the genome comprises a gene encoding an RSV F protein variant or fragment thereof that comprises at least one amino acid substitution compared to a native RSV F protein, wherein the at least one amino acid substitution stabilizes the RSV F protein variant or fragment thereof in a pre-fusion conformation, and wherein the genome comprises at least one additional modification that renders the virus infection-attenuated;
- and
- wherein the cell produces a recombinant, live, attenuated virus of the Pneumoviridae family.
Dependencies are inferred from the claim text.