# US 11,896,369 B2 · Aptamer based portable diagnostic medical device and methods of use > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,896,369 B2 - Document ID: US11896369B2 - Status: Granted - Kind: B2 - Application number: 16/699,534 - Filed: 2019-11-29 - Published: 2020-06-11 - Granted: 2024-02-13 - Earliest claimed: 2018-11-28 - Applicants: Ismael Marvin Voltaire - Inventors: Ismael Marvin Voltaire - Web page: https://patentgrep.com/patent/US11896369B2 ## CPC - A61B 5/14514: {using means for aiding extraction of interstitial fluid, e.g. microneedles or suction} - A61B 5/0022: {Monitoring a patient using a global network, e.g. telephone networks, internet} - A61B 5/0024: {for multiple sensor units attached to the patient, e.g. using a body or personal area network} - A61B 5/14503: {invasive, e.g. introduced into the body by a catheter or needle or using implanted sensors (A61B5/1459, A61B5/1464, A61B5/1473, A61B5/1482, A61B5/14865 take precedence)} - A61B 5/14546: {for measuring analytes not otherwise provided for, e.g. ions, cytochromes} - A61B 5/14556: {by fluorescence (A61B5/14555 takes precedence)} - A61B 5/6824: {Arm or wrist} - A61B 5/6828: {Leg} - A61B: DIAGNOSIS; SURGERY; IDENTIFICATION (usefulness limited to only animals A61D) ## Abstract The present invention is directed to a cuff-like medical device that may analyze and report the concentrations of various biomarkers in a subject's bodily fluids, samples of which may be extracted by the device in the field and analyzed on the spot using the cuff-like medical device. For example, extracellular fluid (commonly known as “tissue fluid”) of a patient may be analyzed by the present invention, and in particular, the constituent interstitial fluid—the known main component of extracellular fluid—may be analyzed for concentrations of one or more specific types of proteins. A sensor component within the device may include one or more aptamers that permit chemical binding of at least one biomarker of interest. When the aptamer-protein binding complex is complete, an electronic component of the device may employ nanoscale weighing using frequency differential analysis through quartz crystal microbalances to determine the presence and concentration of biomarkers. ## Claims ### Claim 1 A device for detecting and measuring one or more biomarker in a biological sample from a subject, the device comprising: a sample access component having at least one microneedle that is adapted to access the biological sample; a sample pre-processing component having at least one first aptamer-based magnetic bead conjugate (“aptamer-MB”), the aptamer-MB having a pre-processing aptamer that is specifically configured to chemically bind to non-desired biomarkers, the non-desired biomarkers being different from the one or more biomarker; wherein the sample pre-processing component further comprises a pre-processing filter wherein the filter has a pore size that is no larger than the largest biomarker being assessed; a sensor component in fluid communication with the sample access component, the sensor component having at least one aptamer that is configured to chemically bind to the one or more biomarker as the biological sample passes through the sensor component, the at least one aptamer being further configured (i) to generate a first resonance frequency prior to being chemically bound to the one or more biomarker and (ii) to generate a second resonance frequency, different from the first resonance frequency, subsequent to being chemically bound to the one or more biomarker; and an electronic component that is configured to collect, analyze, and compute data received from the sensor component, such as the first and second resonance frequencies, in order to determine the presence of the one or more biomarker within the biological sample. ### Claim 2 (depends on 1) The device of claim 1, wherein the sensor component further comprises at least one first aptamer-based quartz crystal microbalance (“aptamer-QCM”) that contains at least two aptamers for detecting the one or more biomarkers. ### Claim 3 (depends on 1) The device of claim 1, wherein the biological sample is interstitial fluid, and wherein the one or more biomarker is a protein contained within the interstitial fluid. ### Claim 4 (depends on 1) The device of claim 1, wherein the at least one microneedle is elongate along a first axis and axially hollow, and wherein the at least one microneedle has two lateral openings on opposed sides thereof that extend into the axially hollow portion in a direction perpendicular to the first axis. ### Claim 5 (depends on 1) The device of claim 1, wherein the electronic component includes a microprocessor that is configured to calculate the presence and concentration of the one or more biomarker from analyzing the first and second resonance frequencies. ### Claim 6 (depends on 5) The device of claim 5, wherein the electronic component further comprises data communication interfaces that enable the device to communicate remotely with computers or devices regarding the detection and measurement of the one or more biomarker. ### Claim 7 (depends on 1) The device of claim 1, wherein the device is configured as a cuff-like device that may be selectively attached to either one of the arm or leg of the subject. ### Claim 8 (depends on 1) The device of claim 1 further comprising: a solution component having at least two vials, the first vial containing a first buffer solution and the second vial containing a second buffer solution that is different than the first buffer solution, the first and second buffer solutions being in selective fluid communication with the sensor component, wherein when at least one of the first and second buffer solutions are selectively put into fluid communication with the sensor component, the at least one buffer solution is configured to chemically dissociate the one or more biomarker from the at least one aptamer. ### Claim 9 (depends on 1) The device of claim 1, wherein the pre-processing filter comprises aptamers configured to bind and remove non-target molecules. ### Claim 10 (depends on 2) The device of claim 2, wherein the sensor component comprises one or more aptamers designated to one or more biomarkers of interest. ### Claim 11 (depends on 2) The device of claim 2, wherein the sensor component comprises two different aptamers designated to each of one or more biomarker of interest. ### Claim 12 (depends on 11) The device of claim 11, wherein the two different aptamers designated to a biomarker of interest are clustered to different sections on the same quartz crystal. ### Claim 13 (depends on 11) The device of claim 11, wherein the two different aptamers are clustered to different quartz crystals. ### Claim 14 (depends on 4) The device of claim 4, wherein the at least one microneedle is equipped with a microneedle filter near the base of the one or more microneedles. ### Claim 15 (depends on 14) The device of claim 14, wherein the microneedle filter comprises a filter mesh configured to selectively obstruct certain molecules by size from entering the interior of the device. ### Claim 16 (depends on 6) The device of claim 6, wherein the electronic component further comprises a GPS receiver. ### Claim 17 (depends on 16) The device of claim 16, wherein the microprocessor is configured to obtain location using a GPS receiver.