# US 11,896,033 B2 · Polypeptides having lysozyme activity, polynucleotides encoding same and uses and compositions thereof > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,896,033 B2 - Document ID: US11896033B2 - Status: Granted - Kind: B2 - Application number: 16/462,764 - Filed: 2017-12-21 - Published: 2021-09-09 - Granted: 2024-02-13 - Earliest claimed: 2016-12-21 - Assignees: Novozymes A/S - Applicants: Novozymes A/S - Inventors: Mikkel Klausen; Kirk Matthew Schnorr; Soeren Nymand-Grarup; Peter Bjarke Olsen; Marianne Thorup Cohn; Robert Piotr Olinski; Marc Dominique Morant; Ming Li; Ye Liu; Lars Kobbeeroee Skov; Dominique Aubert Skovlund; Han Bucong - Web page: https://patentgrep.com/patent/US11896033B2 ## CPC - A23K 20/147: Polymeric derivatives, e.g. peptides or proteins - A23K 10/30: from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms (obtained by microbiological or biochemical processes, e.g. using yeasts or enzymes, A23K10/10) - A23K 20/189: Enzymes - A23K 50/30: for swines - A23K 50/75: for poultry - C12N 9/2462: {Lysozyme (3.2.1.17)} - C12N 15/815: {for yeasts other than Saccharomyces} - A61K 38/00: Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00) - C12Y 302/01017: Lysozyme (3.2.1.17) - Y02P 60/87: Re-use of by-products of food processing for fodder production - A23K: FODDER - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - C12Y: ENZYMES - Y02P: CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS ## Abstract Provided are animal feed or animal feed additives comprising one or more polypeptides having lysozyme activity. Provided are polypeptides having lysozyme activity, polynucleotides encoding the polypeptides nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. ## Claims ### Claim 1 A recombinant host cell comprising a polynucleotide encoding a GH25 polypeptide having lysozyme activity, wherein the polypeptide has at least 80% sequence identity to SEQ ID NO: 9 and the polynucleotide is operably linked to one or more control sequences that direct the production of the polypeptide. ### Claim 2 (depends on 1) The recombinant host cell of claim 1, wherein the polypeptide has at least 85% sequence identity to SEQ ID NO: 9. ### Claim 3 (depends on 1) The recombinant host cell of claim 1, wherein the polypeptide has at least 90% sequence identity to SEQ ID NO: 9. ### Claim 4 (depends on 1) The recombinant host cell of claim 1, wherein the polypeptide has at least 95% sequence identity to SEQ ID NO: 9. ### Claim 5 (depends on 1) The recombinant host cell of claim 1, wherein the polypeptide has at least 97% sequence identity to SEQ ID NO: 9. ### Claim 6 (depends on 1) The recombinant host cell of claim 1, wherein the polypeptide comprises the amino acid sequence of SEQ ID NO: 9. ### Claim 7 (depends on 1) The recombinant host cell of claim 1, wherein the polypeptide is a fragment of SEQ ID NO: 9 and wherein the fragment comprises at least 180 amino acids. ### Claim 8 (depends on 4) The recombinant host cell of claim 4, which comprises more than one copy of the polynucleotide. ### Claim 9 (depends on 8) The recombinant host cell of claim 8, which comprises two, three, four, or five copies of the polynucleotide. ### Claim 10 (depends on 4) The recombinant host cell of claim 4, wherein at least one of the one or more control sequences is heterologous to the polynucleotide. ### Claim 11 (depends on 4) The recombinant host cell of claim 4, which is a yeast cell or a filamentous fungal cell. ### Claim 12 (depends on 11) The recombinant host cell of claim 11, which is an Acremonium, Aspergillus, Aureobasidium, Bjerkandera, Ceriporiopsis, Chrysosporium, Coprinus, Coriolus, Cryptococcus, Fihbasidium, Fusarium, Humicola, Magnaporthe, Mucor, Myceliophthora, Neocallimastix, Neurospora, Paecilomyces, Penicillium, Phanerochaete, Phlebia, Piromyces, Pleurotus, Schizophyllum, Talaromyces, Thermoascus, Thielavia, Tolypocladium, Trametes, or Trichoderma cell. ### Claim 13 (depends on 4) The recombinant host cell of claim 4, which is an Aspergillus awamori, Aspergillus foetidus, Aspergillus fumigatus, Aspergillus japonicus, Aspergillus nidulans, Aspergillus niger, Aspergillus oryzae, Trichoderma harzianum, Trichoderma koningii, Trichoderma longibrachiatum, Trichoderma reesei, or Trichoderma viride cell. ### Claim 14 (depends on 1) A method of producing a GH25 polypeptide having lysozyme activity, comprising (a) cultivating a host cell of claim 1 under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide. ### Claim 15 (depends on 4) A method of producing a GH25 polypeptide having lysozyme activity, comprising (a) cultivating a host cell of claim 4 under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide. ### Claim 16 (depends on 9) A method of producing a GH25 polypeptide having lysozyme activity, comprising (a) cultivating a host cell of claim 9 under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide. ### Claim 17 (depends on 10) A method of producing a GH25 polypeptide having lysozyme activity, comprising (a) cultivating a host cell of claim 10 under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide. ### Claim 18 (depends on 11) A method of producing a GH25 polypeptide having lysozyme activity, comprising (a) cultivating a host cell of claim 11 under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide. ### Claim 19 (depends on 12) A method of producing a GH25 polypeptide having lysozyme activity, comprising (a) cultivating a host cell of claim 12 under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide. ### Claim 20 (depends on 13) A method of producing a GH25 polypeptide having lysozyme activity, comprising (a) cultivating a host cell of claim 13 under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide.