# US 11,896,022 B2 · Interactions between cultures, coagulants and technology to increase cheese yields

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- Number: US 11,896,022 B2
- Document ID: US11896022B2
- Status: Granted
- Kind: B2
- Application number: 16/961,301
- Filed: 2019-01-10
- Published: 2021-03-18
- Granted: 2024-02-13
- Earliest claimed: 2018-01-11
- Assignees: Chr. Hansen A/S
- Applicants: Chr. Hansen A/S
- Inventors: Sebastien Roustel; Véronique Jactat; Ulf Mortensen; Viviana Ester Bruno; Michael Mitsuo Saito
- Web page: https://patentgrep.com/patent/US11896022B2

## CPC

- A23C 19/0684: {Soft uncured Italian cheeses, e.g. Mozarella, Ricotta, Pasta filata cheese; Other similar stretched cheeses}
- A23C 19/0323: {using only lactic acid bacteria, e.g. Pediococcus and Leuconostoc species; Bifidobacteria; Microbial starters in general (using moulds A23C19/0682)}
- A23C: DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF (preservation thereof A23B11/00)

## Abstract

The present invention relates to a process for making low-moisture mozzarella cheese using recent developments in the technical knowledge about the interactions between cultures, coagulants and cheese technology to increase cheese yields and maintain the cheese quality and functionalities. An optimization may lead to a higher pH and higher dry matter of the curd at the whey off step i.e. pH higher than 6.3 and ideally higher than 6.4 and solid non-fact content higher than 18%, without any modification of the curd composition at the stretching step, i.e. pH between 5.0 and 5.3 and more precisely between without any modification of the curd composition at the stretching step, i.e. pH between 5.0 and 5.3 and more precisely between 5.05 and 5.25, Ca/SNF between 1.7% and 2.4% and more precisely between 1.7 and 2.2%, dry matter between 53% and 55% and more precisely between 53.5% and 54.5%. The coagulant has a C/P ratio of at least 25. This optimization may also lead to a reduction in the processing time in the cheese vat (near 15%), so a real increase in the through-put and profitability of the cheese vats.

## Claims

### Claim 1

A process for making a low moisture mozzarella cheese (LMMC), comprising:
A) adding to milk, a starter culture and, optionally, calcium, to obtain a composition;
B) adding one or more coagulants to the composition of step A;
C) renneting the composition of step B for 5 minutes to 25 minutes to obtain a renneted composition;
D) cutting the renneted composition of step C;
E) stirring and scalding the composition of step D while heating the composition to around 41° C.;
F) optionally stirring the composition; and
G) removing whey to obtain a curd
wherein step B of adding one or more coagulants to the composition is conducted no later than 10 minutes, after step A of adding the starter culture to milk,
wherein the pH of the composition is at least 6.3 before step G of removing whey,
wherein the one or more coagulants have a clotting activity/unspecific proteolytic activity (C/P) ratio of at least 25, and
wherein the low moisture mozzarella cheese has one or more of (i) a moisture content of 48% to 50% when measured no later than 24 hours after step D of cutting the renneted composition; (ii) a dry matter content of 50% to 52% when measured no later than 24 hours after step D of cutting the renneted composition; and (iii) a fat per dry matter ratio of 0.40 to 0.55 when measured no later than 24 hours after step D of cutting the renneted composition.

### Claim 2 (depends on 1)

The process according to claim 1, further comprising, after step G, one or more of the following:
reticulating the curd of step G;
cutting the curd of step G;
optionally reticulating the curd a second time;
milling the curd;
salting the curd, and
stretching the curd.

### Claim 3 (depends on 1)

The process according to claim 1, wherein in step A the starter culture is added in an amount from 7.5 g to 15 g per 100 liters of milk.

### Claim 4 (depends on 1)

The process according to claim 1, wherein the starter culture is added as frozen or freeze-dried pellets.

### Claim 5 (depends on 1)

The process according to claim 1, wherein the starter culture comprises at least one protease positive Streptococcus thermophilus strain and optionally one or more of at least one Lactobacillus bulgaricus and at least one Lactobacillus helveticus strain.

### Claim 6 (depends on 1)

The process according to claim 1, wherein the coagulant is a chymosin of camel or bovine origin.

### Claim 7 (depends on 1)

The process according to claim 1, wherein the coagulant has a C/P ratio of at least 30.

### Claim 8 (depends on 1)

The process according to claim 1, wherein the coagulant is added in an amount of 3740 to 5780 IMCU per 100 kg of milk.

### Claim 9 (depends on 1)

The process according to claim 1, wherein the pH of the composition at step C is at least 6.6.

### Claim 10 (depends on 1)

The process according to claim 1, wherein the rennet is added in an amount of 3600 to 4800 IMCU per 100 kg of milk.

### Claim 11 (depends on 1)

A low moisture mozzarella cheese obtained by the process of claim 1.

### Claim 12 (depends on 11)

The cheese according to claim 11, wherein the cheese has a stretchability of at least 1000 mm after 30 days, wherein stretchability is assessed by placing the cheese in a well in a thermostatically controlled water bath at about 90° C. for about 10 minutes, thereby heating the cheese, pulling the heated cheese vertically, and measuring how much the heated cheese stretches before it breaks using a Filometer.

### Claim 13 (depends on 11)

The cheese according to claim 11, wherein the cheese has a ratio of soluble nitrogen to total nitrogen (SN/TN) of at least 3.7 eight days after production.