# US 11,896,012 B2 · Phitosterols suspension concentrates and process of preparation thereof > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,896,012 B2 - Document ID: US11896012B2 - Status: Granted - Kind: B2 - Application number: 17/679,997 - Filed: 2022-02-24 - Published: 2022-09-22 - Granted: 2024-02-13 - Earliest claimed: 2017-06-15 - Assignees: Isagro S.p.A. - Applicants: Isagro S.P.A. - Inventors: Paolo Bellandi; Ilenia Fiorenza Mazzali; Elisa Galimberti; Claudio Dacarro - Web page: https://patentgrep.com/patent/US11896012B2 ## CPC - A01N 45/00: Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring (halogenated hydrocarbons A01N29/08; condensed with heterocyclic rings A01N43/00) - A01N 25/04: Dispersions, {emulsions, suspoemulsions, suspension concentrates} or gels (foams A01N25/16) - A01N 49/00: Biocides, pest repellants or attractants, or plant growth regulators, containing compounds containing the group [IMAGE cpc-sch-A01N-0948.gif], wherein m+n>=1, both X together may also mean —Y— or a direct carbon-to-carbon bond, and the carbon atoms marked with an asterisk are not part of any ring system other than that which may be formed by the atoms X, the carbon atoms in square brackets being part of any acyclic or cyclic structure, or the group [IMAGE cpc-sch-A01N-0949.gif], wherein A means a carbon atom or Y, n>=0, and not more than one of these carbon atoms being a member of the same ring system, e.g. juvenile insect hormones or mimics thereof (containing hydrocarbons A01N27/00) - A01P 21/00: Plant growth regulators - A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS - A01P: BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS ## Abstract Concentrated suspensions containing phytosterols are described, in an amount higher than 25% by weight, in addition to a process for their preparation and their relative use as stimulants of plant growth. ## Claims ### Claim 1 A method of stimulating plant growth comprising: a) preparing a concentrated suspension containing phytosterols, comprising grinding one or more phytosterols until an average particle size of less than 10 pm is reached, said concentrated suspension comprising: i) a phytosterol mixture of beta-sitosterol, stigmasterol, cam pesterol and brassicasterol, in an amount higher than 25% by weight with respect to the total weight of the concentrated suspension, wherein beta-sitosterol is present in an amount ranging from 40% to 45% by weight with respect to the total weight of the mixture of phytosterols, the campesterol is present in an amount ranging from 20% to 25% by weight with respect to the total weight of the mixture of phytosterols, the stigmasterol is present in an amount ranging from 20% to 30% by weight with respect to the total weight of the mixture of phytosterols and the brassicasterol is present in an amount ranging from 1% to 5% by weight with respect to the total weight of the mixture of phytosterols; ii) one or more wetting agents in an amount ranging from 1% to 5% by weight with respect to the total weight of the concentrated suspension; and iii) one or more dispersing agents in an amount ranging from 1% to 5% by weight with respect to the total weight of the concentrated suspension; b) applying the concentrated suspension by one or more of application to any part of a plant, application to the soil by means of fertigation, incorporation into the ground, through seed tanning, and by combinations thereof. ### Claim 2 (depends on 1) The method according to claim 1, wherein the beta-sitosterol is present in an amount equal to about 43% by weight, the campesterol is present in an amount equal to about 24% by weight, the stigmasterol is present in an amount equal to about 26% by weight and the brassicasterol is present in an amount equal to about 2% by weight, with respect to the total weight of the mixture of phytosterols. ### Claim 3 (depends on 1) The method according to claim 1, wherein one or more wetting agents are selected from non-ionic surfactants, anionic surfactants and mixtures thereof. ### Claim 4 (depends on 1) The method according to claim 1, wherein one or more dispersing agents are selected from non-ionic surfactants, anionic surfactants and mixtures thereof. ### Claim 5 (depends on 1) The method of suspension according to claim 1, wherein the grinding of the one or more phytosterols occurs before or after mixture with the one or more wetting agents and one or more dispersing agents, or on the concentrated suspension. ### Claim 6 (depends on 1) The method according to claim 1, comprising applying effective and non-phytotoxic doses of the a concentrated suspension on any part of the plant. ### Claim 7 (depends on 1) The method according to claim 1, wherein the phytosterol mixture of beta-sitosterol, stigmasterol, campesterol and brassicasterol is in an amount ranging from 26% to 40% by weight of the concentrated suspension. ### Claim 8 (depends on 1) The method according to claim 1, wherein the phytosterol mixture of beta-sitosterol, stigmasterol, campesterol and brassicasterol is in an amount ranging from 26% to 35% by weight with respect to the total weight of the concentrated suspension. ### Claim 9 (depends on 1) The method according to claim 1, wherein the one or more wetting agents is in an amount ranging from 2% to 4% by weight with respect to the total weight of the concentrated suspension. ### Claim 10 (depends on 1) The method according to claim 1, wherein the one or more wetting agents is in an amount equal to about 3% by weight with respect to the total weight of the concentrated suspension. ### Claim 11 (depends on 1) The method according to claim 1, wherein the one or more dispersing agents is in an amount ranging from 1.5% to 3% by weight with respect to the total weight of the concentrated suspension. ### Claim 12 (depends on 1) The method according to claim 1, wherein the one or more dispersing agents is equal to about 2% by weight with respect to the total weight of the concentrated suspension. ### Claim 13 (depends on 3) The method according to claim 3, wherein the one or more wetting agents are anionic surfactants. ### Claim 14 (depends on 4) The method according to claim 4, wherein the one or more dispersing agents are anionic surfactants. ### Claim 15 (depends on 5) The method according to claim 5, wherein the suspension is applied by leaf application of the suspension to the plant.