# US 11,896,009 B2 · Bioactive composition for improving stress tolerance of plants

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- Number: US 11,896,009 B2
- Document ID: US11896009B2
- Status: Granted
- Kind: B2
- Application number: 15/760,468
- Filed: 2016-09-15
- Published: 2018-08-30
- Granted: 2024-02-13
- Earliest claimed: 2015-09-15
- Assignees: Fyteko
- Applicants: Fyteko
- Inventors: Juan-Carlos Cabrera Pino; Guillaume Wegria
- Web page: https://patentgrep.com/patent/US11896009B2

## CPC

- A01N 37/38: having at least one oxygen or sulfur atom attached to an aromatic ring system
- A01G 7/06: Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
- A01N 65/00: Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof (containing compounds of determined constitution A01N27/00 - A01N59/00)
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01G: HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING (picking of fruits, vegetables, hops or the like A01D46/00; propagating unicellular algae C12N1/12)

## Abstract

Disclosed is a composition for improving stress tolerance of plants including at least one hydroxycinnamic derivative oligomer, and optionally a water-solubilizing agent. Also disclosed is a method for improving stress tolerance of a plant including applying such composition on the plant.

## Claims

### Claim 1

A composition for improving abiotic stress tolerance in plants comprising:
at least one hydroxycinnamic acid oligomer comprising dimers, tetramers, hexamers, and octamers of hydroxycinnamic acid,
wherein said composition comprises from about 0.01% to about 0.5% of hydroxycinnamic acid monomer, expressed in percent relative to the total hydroxycinnamic acid oligomers of the composition, and
wherein said composition comprises at least 75% of hydroxycinnamic acid dimer, expressed in percent relative to the total hydroxycinnamic acid oligomers of the composition, and
at least one water-solubilizing agent selected from the group consisting of positively or negatively charged polysaccharides and polyols.

### Claim 2 (depends on 1)

The composition according to claim 1, wherein said at least one hydroxycinnamic acid oligomer is selected from the group consisting of oligomer of ferulic acid, oligomer of p-coumaric acid, oligomer of caffeic acid, and oligomer of sinapinic acid.

### Claim 3 (depends on 1)

The composition according to claim 1, wherein said at least one water-solubilizing agent is a positively or negatively charged polysaccharide.

### Claim 4 (depends on 1)

The composition according to claim 1, wherein said at least one water-solubilizing agent is a polyol.

### Claim 5 (depends on 3)

The composition according to claim 3, wherein said polysaccharide is chitosan.

### Claim 6 (depends on 1)

The composition according to claim 1, comprising from 0.01 to 10% of the at least one water-solubilizing agent, expressed in weight percent relative to the total weight of the composition.

### Claim 7 (depends on 5)

The composition according to claim 5, comprising from 0.01 to 10% of the chitosan, expressed in weight percent relative to the total weight of the composition.

### Claim 8 (depends on 1)

A method for improving abiotic stress tolerance of a plant in need thereof; comprising applying to the plant an effective amount of the composition according to claim 1.

### Claim 9 (depends on 2)

A method for improving abiotic stress tolerance of a plant in need thereof comprising applying to the plant an effective amount of the composition according to claim 2.

### Claim 10 (depends on 8)

The method according to claim 8, wherein said at least one hydroxycinnamic acid oligomer is oligomer of ferulic acid.

### Claim 11 (depends on 8)

The method according to claim 8, wherein said composition comprises from 0.0001 to 200 ppm of said at least one hydroxycinnamic acid oligomer.

### Claim 12 (depends on 8)

The method according to claim 8, wherein said composition comprises from 0.001 to 100 ppm of said at least one hydroxycinnamic acid oligomer.

### Claim 13 (depends on 8)

The method according to claim 8, wherein said composition comprises from 0.005 to 50 ppm of said at least one hydroxycinnamic acid oligomer.

### Claim 14 (depends on 8)

The method according to claim 8, wherein said abiotic stress is selected from the group consisting of hydric stress, drought, osmotic stress, thermal stress, nutrient deficiency, and chemical stress generated by metallic or organic pollutant in soil to grow said plant.

### Claim 15 (depends on 1)

A method for improving stress tolerance or for modulating proline content of a plant comprising applying the composition according to claim 1 on said plant.

### Claim 16 (depends on 1)

A seed of plant, wherein said seed is coated with the composition according to claim 1.
