# US 11,896,008 B2 · Use of nootkatone to treat mosquito infestations

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- Number: US 11,896,008 B2
- Document ID: US11896008B2
- Status: Granted
- Kind: B2
- Application number: 17/494,048
- Filed: 2021-10-05
- Published: 2022-01-27
- Granted: 2024-02-13
- Earliest claimed: 2016-09-02
- Assignees: Evolva SA
- Applicants: Evolva SA
- Inventors: Jean Amick; Ryan Murphy
- Web page: https://patentgrep.com/patent/US11896008B2

## CPC

- A01N 35/06: containing keto or thioketo groups as part of a ring, e.g. cyclohexanone, quinone; Derivatives thereof, e.g. ketals
- A01N 25/04: Dispersions, {emulsions, suspoemulsions, suspension concentrates} or gels (foams A01N25/16)
- A01N 25/30: characterised by the surfactants
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS

## Abstract

Compositions and methods for treating and preventing mosquito infestations are disclosed herein. Also disclosed herein are compositions and methods to reduce or prevent the spread of diseases for which mosquitoes may act as a vector.

## Claims

### Claim 1

A method of killing a mosquito larva, comprising:
applying an effective amount of a nootkatone-containing composition to a mosquito breeding site comprising one or more mosquito larvae,
wherein the one or more mosquito larvae are killed by the nootkatone applied to the mosquito breeding site.

### Claim 2 (depends on 1)

The method of claim 1, wherein the effective amount of the nootkatone-containing composition exposes the one or more mosquito larvae to a nootkatone concentration of about 0.01% to 0.03% (v/v) nootkatone at the mosquito breeding site.

### Claim 3 (depends on 1)

The method of claim 1, wherein the one or more mosquito larvae are members of the genera Anopheles, Aedes, Culex, or Haemagogus.

### Claim 4 (depends on 3)

The method of claim 3, wherein the one or more mosquito larvae are Aedes aegypti larvae.

### Claim 5 (depends on 1)

The method of claim 1, wherein the nootkatone is wholly or partially solubilised, liquefied, and/or dispersed in the nootkatone-containing composition.

### Claim 6 (depends on 5)

The method of claim 5, wherein the nootkatone-containing composition comprises ethanol, dichloromethane, ethyl acetate, hexane, an oil, a non-polar solvent, a surfactant, or a mixture or blend thereof.

### Claim 7 (depends on 5)

The method of claim 5, wherein the nootkatone-containing composition comprises a non-toxic, biodegradable nootkatone-solubilizing carrier or surfactant or a mixture or blend thereof.

### Claim 8 (depends on 1)

The method of claim 1, wherein the nootkatone-containing composition is applied to the mosquito breeding site about once per day, about once every 3 days, about once per week, about twice per week, about once per two weeks, about once per month, about once per two months, about once per three months, or about once per season.

### Claim 9 (depends on 8)

The method of claim 8, wherein the nootkatone-containing composition is applied using a dispenser.

### Claim 10 (depends on 9)

The method of claim 9, wherein the dispenser is a pouch, a puck, a pellet, a block, a granule, a vesicle, or a capsule.

### Claim 11 (depends on 9)

The method of claim 9, wherein the dispenser is refillable.

### Claim 12

A method of killing a mosquito pupa, comprising:
applying an effective amount of a nootkatone-containing composition to a mosquito breeding site comprising one or more mosquito pupae, and
wherein the one or more mosquito pupae are killed by the nootkatone applied to the mosquito breeding site.

### Claim 13 (depends on 12)

The method of claim 12, wherein the effective amount of the nootkatone-containing composition exposes the one or more mosquito pupae to a nootkatone concentration of about 0.01% to 0.03% (v/v) nootkatone at the mosquito breeding site.

### Claim 14 (depends on 12)

The method of claim 12, wherein the one or more mosquito pupae are members of the genera Anopheles, Aedes, Culex, or Haemagogus.

### Claim 15 (depends on 14)

The method of claim 14, wherein the one or more mosquito pupae are Aedes aegypti pupae.

### Claim 16 (depends on 12)

The method of claim 12, wherein the nootkatone is wholly or partially solubilised, liquefied, and/or dispersed in the nootkatone-containing composition.

### Claim 17 (depends on 16)

The method of claim 16, wherein the nootkatone-containing composition comprises ethanol, dichloromethane, ethyl acetate, hexane, an oil, a non-polar solvent, a surfactant, or a mixture or blend thereof.

### Claim 18 (depends on 16)

The method of claim 16, wherein the nootkatone-containing composition comprises a non-toxic, biodegradable nootkatone-solubilizing carrier or surfactant or a mixture or blend thereof.

### Claim 19 (depends on 12)

The method of claim 12, wherein the nootkatone-containing composition is applied to the mosquito breeding site about once per day, about once every 3 days, about once per week, about twice per week, about once per two weeks, about once per month, about once per two months, about once per three months, or about once per season.

### Claim 20 (depends on 19)

The method of claim 19, wherein the nootkatone-containing composition is applied using a dispenser.

### Claim 21 (depends on 20)

The method of claim 20, wherein the dispenser is a pouch, a puck, a pellet, a block, a granule, a vesicle, or a capsule.

### Claim 22 (depends on 20)

The method of claim 20, wherein the dispenser is refillable.

### Claim 23

A method of reducing the rate of mosquito egg hatching, comprising:
applying an effective amount of a nootkatone-containing composition to a mosquito breeding site comprising one or more unhatched mosquito eggs,
wherein the rate of mosquito egg hatching of the one or more unhatched mosquito eggs is reduced by the nootkatone applied to the mosquito breeding site.

### Claim 24 (depends on 23)

The method of claim 23, wherein the effective amount of the nootkatone-containing composition exposes the one or more unhatched mosquito eggs to a nootkatone concentration of about 0.01% to 0.03% (v/v) nootkatone at the mosquito breeding site.

### Claim 25 (depends on 23)

The method of claim 23, wherein the one or more unhatched mosquito eggs are members of the genera Anopheles, Aedes, Culex, or Haemagogus.

### Claim 26 (depends on 25)

The method of claim 25, wherein the one or more unhatched mosquito eggs are Aedes aegypti eggs.

### Claim 27 (depends on 23)

The method of claim 23, wherein the nootkatone is wholly or partially solubilised, liquefied, and/or dispersed in the nootkatone-containing composition.

### Claim 28 (depends on 27)

The method of claim 27, wherein the nootkatone-containing composition comprises ethanol, dichloromethane, ethyl acetate, hexane, an oil, a non-polar solvent, a surfactant, or a mixture or blend thereof.

### Claim 29 (depends on 27)

The method of claim 27, wherein the nootkatone-containing composition comprises a non-toxic, biodegradable nootkatone-solubilizing carrier or surfactant or a mixture or blend thereof.

### Claim 30 (depends on 23)

The method of claim 23, wherein the nootkatone-containing composition is applied to the mosquito breeding site about once per day, about once every 3 days, about once per week, about twice per week, about once per two weeks, about once per month, about once per two months, about once per three months, or about once per season.

### Claim 31 (depends on 30)

The method of claim 30, wherein the nootkatone-containing composition is applied using a dispenser.

### Claim 32 (depends on 31)

The method of claim 31, wherein the dispenser is a pouch, a puck, a pellet, a block, a granule, a vesicle, or a capsule.

### Claim 33 (depends on 31)

The method of claim 31, wherein the dispenser is refillable.

### Claim 34

A method of reducing mosquito pupae eclosion, comprising:
applying an effective amount of a nootkatone-containing composition to a mosquito breeding site comprising one or more mosquito pupae,
wherein eclosion of the one or more mosquito pupae is reduced by the nootkatone applied to the mosquito breeding site.

### Claim 35 (depends on 34)

The method of claim 34, wherein the effective amount of the nootkatone-containing composition exposes the one or more mosquito pupae to a nootkatone concentration of about 0.01% to 0.03% (v/v) nootkatone at the mosquito breeding site.

### Claim 36 (depends on 34)

The method of claim 34, wherein the one or more mosquito pupae are members of the genera Anopheles, Aedes, Culex, or Haemagogus.

### Claim 37 (depends on 36)

The method of claim 36, wherein the one or more mosquito pupae are Aedes aegypti pupae.

### Claim 38 (depends on 34)

The method of claim 34, wherein the nootkatone is wholly or partially solubilised, liquefied, and/or dispersed in the nootkatone-containing composition.

### Claim 39 (depends on 38)

The method of claim 38, wherein the nootkatone-containing composition comprises ethanol, dichloromethane, ethyl acetate, hexane, an oil, a non-polar solvent, a surfactant, or a mixture or blend thereof.

### Claim 40 (depends on 38)

The method of claim 38, wherein the nootkatone-containing composition comprises a non-toxic, biodegradable nootkatone-solubilizing carrier or surfactant or a mixture or blend thereof.

### Claim 41 (depends on 34)

The method of claim 34, wherein the nootkatone-containing composition is applied to the mosquito breeding site about once per day, about once every 3 days, about once per week, about twice per week, about once per two weeks, about once per month, about once per two months, about once per three months, or about once per season.

### Claim 42 (depends on 41)

The method of claim 41, wherein the nootkatone-containing composition is applied using a dispenser.

### Claim 43 (depends on 42)

The method of claim 42, wherein the dispenser is a pouch, a puck, a pellet, a block, a granule, a vesicle, or a capsule.

### Claim 44 (depends on 42)

The method of claim 42, wherein the dispenser is refillable.