# US 11,895,964 B2 · Inbred rice line DG263L

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- Number: US 11,895,964 B2
- Document ID: US11895964B2
- Status: Granted
- Kind: B2
- Application number: 17/900,597
- Filed: 2022-08-31
- Published: 2023-07-20
- Granted: 2024-02-13
- Earliest claimed: 2021-06-02
- Assignees: Nutrien AG Solutions, Inc.
- Applicants: Nutrien AG Solutions, Inc.
- Inventors: Qiming Shao; Nanyen Chou; Kirk Douglas Johnson
- Web page: https://patentgrep.com/patent/US11895964B2

## CPC

- A01H 6/4636: {Oryza sp. [rice]}
- A01H 5/10: Seeds
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES

## Abstract

The inbred rice line designated DG263L is disclosed. Embodiments include the seeds of inbred rice line designated DG263L, the plants of inbred rice line designated DG263L, plant parts of inbred rice line designated DG263L, and methods for producing a rice plant produced by crossing rice DG263L with itself or with another rice variety. Embodiments include methods for producing a rice plant containing in its genetic material one or more genes or transgenes and the transgenic rice plants and plant parts produced by those methods. Embodiments also relate to rice cultivars, breeding cultivars, plant parts, and cells derived from inbred rice line designated DG263L, methods for producing other rice cultivars, lines or plant parts derived from inbred rice line designated DG263L, and the rice plants, varieties, and their parts derived from use of those methods. Embodiments further include hybrid rice seeds, plants, and plant parts produced by crossing DG263L with another rice cultivar.

## Claims

### Claim 1

A seed of inbred rice line DG263L, wherein a representative sample of seed of DG263L has been deposited with the National Laboratory for Genetic Resources Preservation (NLGRP) under number PI 695413.

### Claim 2 (depends on 1)

A rice plant or a plant part thereof, produced by growing the rice seed of claim 1.

### Claim 3

A rice plant, or a plant part thereof, having the physiological and morphological characteristics of inbred rice line DGL263L, wherein a representative sample of seed of DG263L National Laboratory for Genetic Resources Preservation (NLGRP) under number PI 695413.

### Claim 4 (depends on 2)

A tissue culture of cells produced from the rice plant part of claim 2, wherein the plant part is selected from the group consisting of pollen, embryos, cotyledon, hypocotyl, meristematic cells, roots, root tips, pistils, anthers, glumes, panicles, flowers, leaves and stems.

### Claim 5 (depends on 4)

A rice plant regenerated from the tissue culture of claim 4 wherein the regenerated plant has all of the morphological and physiological characteristics of inbred rice line DG263L.

### Claim 6 (depends on 2)

A protoplast produced from the plant or plant part of claim 2.

### Claim 7 (depends on 6)

A rice plant regenerated from the protoplast of claim 6 wherein the regenerated plant has all of the morphological and physiological characteristics of inbred rice line DG263L.

### Claim 8 (depends on 3)

A method for producing a F1 hybrid rice seed, comprising crossing the rice plant of claim 3 with a rice plant of a different rice line and harvesting resultant rice seed.

### Claim 9 (depends on 8)

A hybrid rice seed produced by the method of claim 8.

### Claim 10 (depends on 9)

A hybrid rice plant, produced by growing the seed of claim 9.

### Claim 11

A method of introducing one or more desired traits into inbred rice line DG263L, wherein the method comprises:
(a) crossing a rice plant of inbred rice line DG263L with a rice plant of another rice line that comprises the one or more desired traits to produce progeny plants, wherein a representative sample of seed of the inbred rice line DG263L has been deposited with the National Laboratory for Genetic Resources Preservation (NLGRP) under number PI 695413;
(b) selecting one or more progeny plants that have the one or more desired traits to produce selected progeny plants;
(c) backcrossing the selected progeny plants with rice plants of inbred rice line DG263L;
(d) selecting for backcross progeny plants that have the one or more desired traits; and
(e) repeating steps (c) and (d) one or more times in succession to produce selected second or higher backcross progeny plants that comprise the one or more desired traits and the physiological and morphological characteristics of inbred rice line DG263L.

### Claim 12 (depends on 11)

The method of claim 11, wherein the one or more desired traits is one or more of: herbicide resistance; bacterial disease resistance; viral disease resistance; fungal disease resistance; insect resistance; modified fatty acid metabolism; modified carbohydrate metabolism; and male sterility.

### Claim 13 (depends on 11)

A rice plant produced by the method of claim 11.

### Claim 14 (depends on 13)

A rice seed harvested from the rice plant of claim 13, wherein a rice plant grown from the rice seed comprises the morphological and physiological characteristics of inbred rice line DG263L.

### Claim 15 (depends on 2)

A method for producing inbred rice line DG263L seed, comprising crossing a first inbred parent rice plant with a second inbred parent rice plant and harvesting resultant rice seed, wherein both the first and the second inbred parent rice plants are the rice plant of claim 2.

### Claim 16 (depends on 15)

A method for producing a hybrid rice seed, comprising crossing the rice plant of claim 15 with a different rice plant of a different rice line and harvesting resultant rice seed.

### Claim 17 (depends on 16)

A hybrid rice seed produced by the method of claim 16, wherein a rice plant grown from the hybrid rice seed comprises the morphological and physiological characteristics of inbred rice line DG263L.

### Claim 18 (depends on 2)

A method comprising crossing the rice plant of claim 2 with either i) a second rice plant of another rice line which includes a transgene or ii) a genomic edited rice plant of inbred rice line DG263L to produce a progeny plant comprising the edited gene or the transgene wherein the transgene is operably linked to a regulatory element in the progeny plant.

### Claim 19 (depends on 18)

The method of claim 18, wherein the transgene or the edited gene confers to the progeny plant at least one of: herbicide resistance; bacterial disease resistance;
viral disease resistance; fungal disease resistance; insect resistance; modified fatty acid metabolism; modified carbohydrate metabolism; and male sterility.

### Claim 20 (depends on 18)

A rice plant or a part thereof, produced by the method of claim 18 wherein the rice plant comprises the transgene or edited gene and otherwise has all of the morphological and physiological characteristics of inbred rice line DG263L.

### Claim 21 (depends on 2)

A commodity plant product derived or produced from a plant of claim 2.

### Claim 22 (depends on 21)

The commodity plant product of claim 21, wherein the commodity plant product comprises at least one cell of a rice plant of inbred rice line DG263L.

### Claim 23 (depends on 21)

The commodity plant product of claim 21, wherein the commodity plant product is rice, rice meal, rice flour, rice oil, a film, a packaging, or a nutraceutical product.
