# US 11,895,962 B2 · Method for producing agave cultures for tequila

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 11,895,962 B2
- Document ID: US11895962B2
- Status: Granted
- Kind: B2
- Application number: 17/853,163
- Filed: 2022-06-29
- Published: 2023-08-24
- Granted: 2024-02-13
- Earliest claimed: 2022-02-22
- Assignees: Phytoteq, Inc.
- Applicants: Phytoteq, Inc.
- Inventors: Peter Andrew Whitton; John R. Munoz; Geoffrey Dixon
- Web page: https://patentgrep.com/patent/US11895962B2

## CPC

- A01H 4/005: {Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques}
- C12G 3/02: by fermentation
- C12N 5/0025: {Culture media for plant cell or plant tissue culture}
- C12N 5/04: Plant cells or tissues {(culture media C12N5/0025)}
- C12N 2500/34: Sugars
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- C12G: WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES (beer C12C); PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

A method to expedite the growth of the agave plant by growing the plant tissue from a cell culture in a laboratory utilize incubator cells that are taken from the leaf of an agave plant and grown in a medium containing all the nutrients required for the production of new cells and the maturation of those new cells. The cells are then exposed to photosynthetic active radiation and fed with extra carbon dioxide, causing the cells to undergo photosynthesis and form the sugars that are found in a naturally growing agave plant. These cultivated cells are given an optimum amount of light and carbon dioxide to promote an unexpected and substantially higher growth rate than heretofore achieved.

## Claims

### Claim 1

A method for cultivating agave cells for the product of tequila, comprising:
(a) prepare a callus introduction media including sucrose, naphthalene acetic acid, and water;
(b) place agave cells into the introduction media to incubate a callus formation;
(c) establish a mixture by placing the callus formation into an accelerator media including sucrose, naphthalene acetic acid, and water, supplemented by a vitamin stock solution, and modify the mixture to obtain a pH of between 5.5 and 6;
(d) heat the mixture of (c);
(e) use cultures obtained from the mixture of (d) to cultivate subcultures from accelerator media and supplement with citric acid to obtain a pH of between 3.5 and 4.5, and introduce light to the subcultures;
(f) add carbon dioxide and yeast, and ferment;
whereupon an ethanol concentration is adjusted to produce tequila.

### Claim 2 (depends on 1)

The method for cultivating agave cells for the product of tequila of claim 1, wherein the callus introduction media includes Murashige and Skoog medium.

### Claim 3 (depends on 1)

The method for cultivating agave cells for the production of tequila of claim 1, wherein the callus introduction media has a 3:1 ratio of sucrose to naphthalene acetic acid.

### Claim 4 (depends on 1)

The method for cultivating agave cells for the production of tequila of claim 1, wherein the callus introduction media is solidified with phytagel.

### Claim 5 (depends on 1)

The method for cultivating agave cells for the production of tequila of claim 1, further comprising sterilizing the agave prior to introducing the cells to the callus introduction media.

### Claim 6 (depends on 1)

The method for cultivating agave cells for the production of tequila of claim 1, wherein the agave cells and the callus introduction media are sealed in a container and placed in darkness.

### Claim 7 (depends on 1)

The method for cultivating agave cells for the production of tequila of claim 1, wherein the vitamin stock solution comprises pyridoxalhydrochlorid, thiamine dichloride, and nitric acid.

### Claim 8 (depends on 1)

The method for cultivating agave cells for the production of tequila of claim 1, wherein a ratio of cultures to accelerator media in step (e) is 5 grams to 50 mL.

### Claim 9 (depends on 1)

The method for cultivating agave cells for the production of tequila of claim 1, wherein one watt of light is used for each liter of culture liquid.

### Claim 10 (depends on 1)

The method for cultivating agave cells for the production of tequila of claim 1, wherein a total sugars when measured by dry weight is no less than six percent (6%).