Treatment efficacy prediction systems and methods
AssigneeOurotech, Inc.
InventorsDuleeka Nimantha Bandara Ranatunga, Eleonora Peerani, Gastón Agustín Primo, Zhi Yuan Lin, Sacha Hu, Aston Martin Crawley
Life of this patent
Estimating the term…
Abstract
Systems and methods for predicting a patient response to various agents and/or combinations of agents using ex vivo dosing and imaging are disclosed. In one example, a method of determining treatment efficacy includes analyzing a solid cell culture over time, e.g., first and second responses to a solid cell culture to respective treatments may be compared to determine a treatment efficacy of each treatment. Systems and methods for applying the treatments to the cell culture and analyzing the cell culture and efficacy are disclosed.
Claims29
A method of analyzing an ex vivo solid cell culture over time, the method comprising:
- determining a first response of a solid cell culture to a growth media including a treatment agent, the solid cell culture held in a cell culture chamber of a microfluidic chip by determining first location coordinates of at least a first single cell of the solid cell culture including a first x location coordinate, a first y location coordinate, and a first z location coordinate;
- determining a second response of the solid cell culture to the growth media by determining second location coordinates of the at least first single cell of the solid cell culture including a second x location coordinate, a second y location coordinate, and a second z location coordinate;
- and
- comparing the first response and the second response to determine a treatment efficacy,
- wherein comparing the first response and the second response comprises comparing the first location coordinates to the second location coordinates to determine a difference between the first location coordinates and the second location coordinates, and wherein the difference between the first location coordinates and the second location coordinates is between at least one of the first x location coordinate and the second x location coordinate, the first y location coordinate and the second y location coordinate, or the first z location coordinate and the second z location coordinate, the difference representing a cell migration distance of the at least first single cell over time.
The method of The method of wherein determining the cell migration distance of the at least first single cell over time includes comparing the first image and the second image, and the method further comprises predicting a patient response to the treatment agent using the cell migration distance.
Dependencies are inferred from the claim text.