Method for extraction and determination of fatty acid in biological fluid

A method for extraction and determination of fatty acids in a biological fluid sample containing albumin, comprising the following steps;

• adding the biological fluid sample of a patient into a holder containing at least two electrodes, wherein one electrode of the electrodes is a carbon-based electrode with a high surface area,
• performing an incubation of the biological fluid sample in the holder in a predetermined period to increase a contact probability between the albumin in the biological fluid sample and the carbon-based electrode,
• performing an extraction-adsorption process during the incubation, wherein the extraction-adsorption process comprises an concomitant self-extraction of the fatty acids from the albumin and an adsorption of the fatty acids extracted onto a surface of the carbon-based electrode, due to a hydrophobic interaction between the fatty acids and a material of the carbon-based electrode,
• changing an electrode-electrolyte interfacial construct, while the extraction-adsorption process creates a layer of the fatty acids on the carbon-based electrode, wherein as the extraction-adsorption process goes, the electrode-electrolyte interfacial construct changes to form an electrical double layer,
• obtaining data with an electrical/electrochemical technique sensitive to changes of the electrode-electrolyte interfacial construct, wherein the changes of the electrode-electrolyte interfacial construct reflect a magnitude of the fatty acids extracted from the albumin,
• interpretating results from the electrical/electrochemical technique by comparing initial data of a normal individual and/or the patient at a beginning of the incubation with last data of the normal individual and/or the patient at an end of the incubation.