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US 11,892,458 B2Granted
Biochemical predictors of red blood cell quality
AssigneeBloodworks NW
InventorJames Charles Zimring
Life of this patent
Estimating the term…
Abstract
Compositions and methods for determining post-transfusion survival or toxicity of red blood cells and the suitability of red blood cell units for transfusion by measuring the levels of one or more compounds in a red blood cell sample are provided.
Claims12
A method for preparing a red blood cell (RBC) sample useful for determining RBC storage quality, the method comprising:
- obtaining a sample of stored blood from a blood donor, wherein the sample comprises at least one marker selected from the group consisting of aspartate, 2′-deoxyuridine, deoxycarnitine, glycylleucine, trans-4-hydroxyproline, thymidine, isoleucylglycine, gamma-glutamyltyrosine, valylglycine, N6-acetyllysine, glutamine, N-acetyltaurine, N-acetylglycine, 5,6-dihydrothymine, glycerol 3-phosphate, alpha-ketoglutarate, stearate, 1-oleoylglycerol, linoleate, eicosenoate, 10-heptadecenoate, dihomo-linoleate, 4-hydroxy-nonenal-glutathione, 20a-dihydroprogesterone, margarate, 2-phosphoglycerate, oleate, linolenate [alpha or gamma;
- (18:3n3 or 6)], cis-vaccenate, 10-nonadecenoate, succinylcarnitine, 3-methyl-2-oxovalerate, and 2-oleoylglycerol;
- contacting the sample with a reagent to generate a complex between the reagent and the at least one marker;
- detecting the complex between the reagent and the at least one marker;
- and
- measuring a level for the at least one marker, wherein the level of the at least one marker is positively correlated or negatively correlated with RBC storage quality.
3 more elements
A method for preparing a red blood cell (RBC) sample useful for determining RBC storage quality, the method comprising:
- obtaining a sample of stored blood from a blood donor, wherein the sample comprises at least one marker selected from the group consisting of aspartate, glycylleucine, trans-4-hydroxyproline, thymidine, isoleucylglycine, gamma-glutamyltyrosine, valylglycine, N6-acetyllysine, glutamine, and N-acetylglycine;
- contacting the sample with a reagent to generate a complex between the reagent and the at least one marker;
- detecting the complex between the reagent and the at least one marker;
- and
- measuring a level for the at least one marker, wherein the level of the at least one marker is positively correlated or negatively correlated with RBC storage quality.
2 more elements
Dependencies are inferred from the claim text.