Reflective depth interrogation for analyte measurement in liquids
AssigneeAirware, Inc.
InventorsThomas G Campbell, Jacob Y Wong
Life of this patent
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Abstract
An absorption spectroscopy process uses a single radiation beam with two or more pulsed beams (including at least a signal beam and a reference beam) that are passed into a liquid sample to a variable effective depth and then reflected out of the liquid sample where it is detected and processed to obtain a value over a preselected time. As values are determined for multiple effective depths, a sampling dataset is obtained which is used to calculate a concentration level of a targeted particle in the liquid sample by use of calibration dataset obtained from use of known samples.
Claims20
A process for quantifying a concentration of a targeted molecule in a liquid sample through use of an absorption spectroscopy process, comprising:
- (1) pulsing a signal beam with a signal pulsed source;
- (2) pulsing a reference beam with a reference pulsed source;
- (3) spatially combining the pulsed signal beam and the pulsed reference beam into a single radiation beam which passes into the liquid sample to a first effective depth and then is reflected out of the liquid sample;
- (4) detecting a pulsed signal beam output and a pulsed reference beam output after the single radiation beam passes out of the liquid sample;
- (5) processing the pulsed signal beam output and the pulsed reference beam output to obtain a value over a preselected period of time;
- (6) repeating steps (1) through (5) for at least one additional effective depth to obtain a sampling dataset;
- and
- (7) calculating a concentration level of the targeted particle in the liquid sample based on the sampling dataset;
- wherein the signal pulsed source emits radiation at the signal bandwidth which is coincident with an absorption band of the targeted particle while the reference pulsed source emits radiation at a reference wavelength which is not coincident with the absorption band;
- and
- wherein the signal pulsed source is separate from the reference pulsed source.
An apparatus for quantifying a concentration of a targeted molecule in a liquid sample, comprising:
- a device configured to use an absorption spectroscopy process comprising the following steps:
- (1) pulsing a signal beam with a signal pulsed source;
- (2) pulsing a reference beam with a reference pulsed source;
- (3) spatially combining the pulsed signal beam and the pulsed reference beam into a single radiation beam which passes into the liquid sample to a first effective depth and then is reflected out of the liquid sample;
- (4) detecting a pulsed signal beam output and a pulsed reference beam output after the single radiation beam passes out of the liquid sample;
- (5) processing the pulsed signal beam output and the pulsed reference beam output to obtain a value over a preselected period of time;
- (6) repeating steps (1) through (5) for at least one additional effective depth to obtain a sampling dataset;
- and
- (7) calculating a concentration level of the targeted particle in the liquid sample based on the sampling dataset;
- wherein the signal pulsed source emits radiation at the signal bandwidth which is coincident with an absorption band of the targeted particle while the reference pulsed source emits radiation at a reference wavelength which is not coincident with the absorption band;
- and
- wherein the signal pulsed source is separate from the reference pulsed source.
Dependencies are inferred from the claim text.