Methods for generating therapeutic delivery platforms
AssigneesUniversity of Kansas, Kansas State University Research Foundation
InventorMei He
Life of this patent
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Abstract
Methods for producing engineered exosomes and other vesicle-like biological targets, including allowing a target vesicle-like structure to react and bind with immunomagnetic particles; capturing the immunomagnetic particle/vesicle complex by applying a magnetic field; further engineering the captured vesicles by surface modifying with additional active moieties or internally loading with active agents; and releasing the engineered vesicle-like structures, such as by photolytically cleaving a linkage between the particle and engineered vesicle-like structures, thereby releasing intact vesicle-like structures which can act as delivery vehicles for therapeutic treatments.
Claims19
A composition for isolating an extracellular vesicle, the composition comprising a plurality of magnetic particles, each magnetic particle comprising:
- a core particle, wherein said core particle is coated with a layer comprising graphene-oxide nanosheets covalently bonded to the core particle, further comprising at least one polydopamine polymer coupled with the graphene-oxide nanosheets;
- and
- a targeting moiety extending from the core particle via a photocleavable linker, wherein said targeting moiety is coupled to at least one polydopamine polymer, and said photocleavable linker connecting the magnetic particle and the targeting moiety, wherein the targeting moiety specifically binds to an extracellular vesicle surface marker for isolating said extracellular vesicle.
Dependencies are inferred from the claim text.